Functional Investigation Of Putative Chm1 Effectors In Magnaporthe Oryzae

Magnaporthe oryzae is one of the model organisms for studying the interaction between fungi and plant,its conidium is the important structure for disease dissemination,whereas the mechanisms of conidiation are still unclear.Small GTPase MoRacl is one member of the Rho family proteins,and both MoRacl and its downstream effector Chm1 regulates the conidia formation in M.oryzae.Based on the results of RNA-seq and relevant study in yeast,we chose three putative Chm1 effectors MoUbr 1?MGG₀0409?,MoFre3?MGG 07833?and MoSep3?MGG₀1521?for further study.Phenotypes analysis indicates that the growth rate,conidiation,septal formation and pathogenicity of ?Mosep3 were more or less affected,similare to those of Achml,On the other hand,deletion of MoUbrl or MoFre3 did not show any obvious phentype.The pull down and sub-cellular localization assays showed that MoSep3 indeed interacted with Chml.MoSep3 involved in the process of septum formation and then might sujected to be degraded in the vacuole.We also found that Chml is mostly distributed in the cytoplasm and aggregated at the basal part of conidia.All the above suggest that MoSep3 and Chml play significant roles in vegetative growth and pathogenicity and then they co-regulate conidia formation in M.oryzae.