| Bradysia impatiens is the main fungi pest,which has seriously threatened sustainable development of fungus industry worldwide and occupied a wide variety of ecological niches.It was commonly believed that the larvae fed exclusively upon dead organic matter and fungi,but it was observed attacked plants even when the seedlings seemly healthy.The purpose of this paper was to find out what special funguses that attract B.impatiens at laboratory,whether larvae actually attack healthy plants.The results are summarized as follows:(1)There are four isolated funguses obtained from laboratory.Identification of isolate fungi by NCBI Blast of ITS sequence demonstrated they were high similar with Aspergillus flavus,Mucor circinelloides,Rhizopus oryzae and Fusarium solani.Phylogenetic tree of isolate fungi are all in 100% confidence.(2)The attraction of four isolate funguses which has separately cultured 7 days to B.impatiens were studied,the results showed that only A.flavus and M.circinelloides had significantly attracted adults and also varied greatly between genders.(3)A design of fungal diets vs non–fungal diets to test larval survival and development time.The mean survival on the fungal diets was 88% and 3%,respectively.Larvae on non–fungal diets were no survived to adult stage while there was 85% survival on control.The mean developmental time on fungal diets was 1.9 d earlier than on control.(4)Behavioral responses in a four–choice olfactometer to six edible fungus hosts showed either fruit body,culture medium or hyphae B.impatiens were high tropism to Pleurotus ostreatus.(5)Data from the four–choice olfactometer experiment showed a significant difference in the behavioral responses of B.impatiens females and males to the treatments.Both sexes were significantly more attracted to M.circinelloides than to the Pleurotus ostreatus hyphae or A.flavus suggesting that M.circinelloides significantly attracted B.impatiens. |
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