Entomogenous fungi are classified as fungi that grow either inside or on the surface of insect bodies,which eventually cause the death of the host insect.Entomogenous fungus is a rich source of natural bioactive compounds.In recent years,some lead compounds found in the metabolites of entomogenous fungus Aschersonia and its teleomorph that have anti-tumor,anti-bacterial,anti-inflammatory,and insecticidal activity.In order to search for novel bioactive natural products,we have investigated chemical constituents and bioactivities of the metabolites from Aschersonia WYTY-21 and WYTY-23.On the basis of a series of chromatographic techniques including TLC,ODS,Sephadex LH-20 and R-HPLC,twelve compounds involved in two new and seven firstly isolated compounds were obtained from the broth(filtrate)and mycelia of Aschersonia.Compounds including 5a,8a-epidioxy-24(R)-methylcholesta-6,22-diene-3?-ol(1*),dustanin(2*),3?-acetoxy-15a,22-dihydroxyhopane(3*),ergosterol(4*),and 7?,15a,22-trihydroxyhopane(5*)were isolated from WYTY-23.The other metabolites containing(7R,8R,8aS)-5-chlor-3-((3R,4R,5S,E)-3,4-dihydroxy-3,5-dimet hylhept-1-enyl)-7,8-dihydroxy-7-methyl-8,8a-dihydro-1H-isochromen-6(7H)-one(6*),(7R,8R,8aR)-5-chloro-3-((3R,4R,5S,E)-3,4-dihydroxy-3,5-dimethylhept-l-enyl)-7-hy droxy-7-methyl-6-oxo-6,7,8,8a-tetrahydro-lH-isochromen-8-ylacetate(7*),(7R,8R,8a R)-5-chloro-3-((3R,4R,5S,E)-3,4-dihydroxy-3,5-dimethylhept-l-enyl)-7,8-dihydroxy-7-methyl-8,8a-dihydro-1H-isochromen-6(7H)-one(8*),5-chloro-3-((3R,4R,5S,E)-3,4-dihydroxy-3,5-dimethylhept-l-enyl)-7,8-dihydroxy-7-methyl-7,8-dihydro-6H-isochro men-6-one(9*),isochromophilon ?(10*),sclerotioramine(11*)and flufuran(12*)were originated from WYTY-21.In this study,the structure of the isolated compounds was elucidated by extensive spectroscopic methods containing NMR,EIMS,HREIMS,CD,Mosher,and so forth.MTT assay method was used to conduct in vitro anti-tumor activity screening of monomeric compounds.The results showed that the compounds(1*?12*)displayed different inhibitory activity on the growth of human hepatoma cell BEL-7404,but expressed no cytotoxicity to two strains of normal cells(human liver cells HL-7702 and human kidney epithelial cell HEK-293T).Among them,compouds 7*,8*,10*,11*,indicated a significant inhibition effect on BEL-7404 with an IC50 value of 17.4?mol/L,16.1?mnol/L,6.2?mol/L,11.6 ?mol/L,respectively.Moreover,compounds 1*,10*,11*showed a significant inhibitory activity to human gastric cancer cells SGC-7901,with IC50 values of 56.1 ?mol/L,14.3 ?mol/L,18.5?mol/L,respectively. |