Development Of High G Loading Device And Finite Element Simulation Of Single Cell

With the rapid development of modern weapons and scientific equipment,modern fighters are becoming more and more powerful,pilots and astronauts will have more and more opportunities in the high acceleration environment because more and more manned space activities,a sustained high acceleration(high G)environment can have a major impact on human function.The academic community has had little research on this effect because the materials are difficult to obtain.The researchers use small animals as research materials,let them be in a high G environment for a time,through experimental analysis to study the effects of high G environment on their bodies,tissues and cells.In this thesis,the types and development of centrifuges are reviewed.Through study and analysis,the loading device provide a controllable high G environment for animal and cell loading experiments was developed.The loading machine is mainly composed of a base,a transmission mechanism,a rotor,a servo motor,a control box,and an outer casing.During the design process,the modal analysis of the base part using finite element software is carried out to avoid resonance of the base when the machine is working;various rotor structures are discussed,and finally the rectangular steel with stable structure and easy processing is used to make the rotor;through the calculation,a cost-effective servo motor was selected,and a text display and PLC which are convenient for programming and use were selected as the control module.The main performance parameters of the machine: effective radius of rotor is 40 cm,rated G value is 40 G,maximum G value change rate is 6.7 G/s,and two rats can be loaded at the same time.When the loading machine was completed,we carried out the animal loading pre-experiment and group experiment,verified the loading effect of the loading machine,and observed the changes of the body and mobility of the rats after loading in different high G mechanical environments.Researching the effects of high G mechanics on the body and mobility of animals.In addition,the equipment designed and manufactured in this paper can provide a high G mechanical environment for the cells through the clamping device in addition to animal loading.We have observed that the shape of the osteoblasts after high G loading will change.In order to further study the stress of the cells in the high G environment,we established a cell model containing the cytoskeleton for finite element analysis.Before modeling,we firstly sorts the dimensions of Abaqus software,and sorts out the unit system using m,mm,and ?m as the unit of length.The model is verified in software to determine each unit system is right.Then,modeling the single cell model,and the types of cell finite element models established by many scholars were reviewed.The advantages and disadvantages of the cells were summarized.The finite element model with cytoskeleton was selected.According to the research of cell materials,the appropriate size parameters and material parameters are selected to establish a cell model.Add different sizes of high-gravity G to the cells,and analyze the contains or absence of cytoskeletal cell models after high G-load loading,It is clear that the cytoskeleton plays a role in resisting the high G force of the cells and maintaining the shape of the cells,and this model can predict various responses and changes of cells in the high G mechanical environment loading experiment.The work of this paper is mainly to provide a loading device for high G loading of laboratory animals;A rat loading experiment was performed to analyze the effects of high G mechanical environment on the mobility and growth of rats;Establishing a three-dimensional model of cells containing cytoskeleton,It lays a good foundation for the future development of cell finite element simulation in high G environment.