Imaging System Design And Vibration Analysis Of Single Molecular Gene Sequencer

With the improvement of living standards,people pay more and more attention to their own life and health.Since the emergence of the concept of accurate medical treatment,the domestic downstream gene sequencing companies have grown explosively,and the demand for upstream gene sequencers has become greater.The single-molecule gene sequencer designed in this paper adopts the method of edge sequencing to sequence the interrupted DNA molecules directly,which is very suitable for clinical applications.It uses the total internal reflection fluorescence microscopy to detect extremely small and fragile DNA single-molecule fluorescence signals,so the sequencer is very sensitive to vibration.Therefore,this paper focuses on the research of vibration suppression technology,vibration isolation system design and vibration evaluation scheme for the characteristics of the single-molecule gene sequencer system to effectively isolate and attenuate the interference of external and internal vibration factors,so as to ensure that the sequencing process has a clear and stable single-molecule image,which guarantees the accuracy and reliability of sequencing results.According to the requirement and function analysis of single molecule gene sequencer,the imaging system was constructed,and the functional modules are designed.Three different structural models were designed by the Solidworks.The composition,working principle and the unusual sensitivity to vibration of the optical system were analyzed.According to the imaging principle,a set of vibration evaluation scheme was designed as the basis for the further verification of test experiment.The vibration excitation source of the sequencer was studied,the system dynamics model was established based on vibration the theory to facilitate the observation of the essential law of vibration suppression,and the vibration suppression optimization method was studied.The response characteristics of the imaging system under external and internal excitation were analyzed.The external excitation in the main environment was tested experimentally,and the internal excitation of the working state was analyzed according to the working conditions.In additional,the parameters of the vibration isolation effect were also studied.The models of the imaging systems of the three monolayer sequencers were designed and compared,with corresponding finite element models established by the Ansys.By analyzing the first ten-order modes of vibration,the first-order natural frequencies of the X / Y / Z directions of the corresponding models were obtained.Based on the theoretical analysis and simulation results,the optimal structure model was acquired through the overall numerical comparison with the diaphragm data.The experimental test platform was built,on which the image processing and data processing were completed,and the experimental test analysis was carried out.The vibration suppression effects of the three different structures were compared with the finite element analytic results,which confirmed the structure vibration suppression effect.