Inactivation Of Enterovirus By Ferrate And Peracetic Acid

Enterovirus is one of the major viruses in drinking water resource and sewage,and it is the main reason to cause human and animal diarrhea.Surface water is an important resource for most drinking water plants in China.However,due to the high content of nitrogen and phosphorus,surface water experiences frequent algae bloom.In this paper,the inactivation of the bacteriophage MS2 by a clean disinfectant ferrate under different pH conditions was studied.We also studied the inactivation rate kinetics of different ferrate species to MS2 under different pH conditions.When the raw water is contaminated by algae,the influence of the algal organic matter?AOM?released by the algae cells includes the Extracellular Organic Matter?EOM?and the Intracellular Organic Matter?IOM?to MS2 inactivation was also studed.Moreover,the inactivation of rotavirus OSU and circovirus TV by another environmentally friendly green oxidant,peracetic acid?PAA?was investigated.We studied the aggregation of rotavirus OSU and circovirus TV under different pH conditions and the impact of aggregation on inactivation of OSU and TV by PAA.The main conclusions obtained are as follows:?1?Under different pH conditions,ferrate has different forms of existence which have different rates to inactivate MS2.The inactivation rates of two species of ferrate H₂FeO₄ and HFeO₄^-to MS2 inactivation were 1935 and 8 times than that of FeO₄^2-,respectively.When pH=7,the main contributions to MS2 inactivation are HFeO₄^-?36%?and H₂FeO₄?59%?;when pH=8,HFeO₄^-?54%?and FeO₄^2-?41%?are main contributors to MS2 inactivation,while the contribution of H₂FeO₄ reduced to 5%,because it is only 5×10^-4%in total Fe???;and when pH=8.7,The contribution of FeO₄^2-to MS2 inactivation is 79%,and the contribution of HFeO₄^-to inactivated MS2 is 20%.?2?Both IOM and EOM promoted the decay of Fe???.Under the same pH conditions,IOM had a greater effect on the decay of Fe???,which may due to the difference in IOM and EOM composition.It can be seen from the EEM spectrum that the phycocyanin contained in the IOM had antioxidant properties which can react quickly with Fe???,and the reaction was stronger than the humic acid and fulvic acid contained in the EOM.?3?In the MS2 inactivation experiments,the algae organic matter EOM and IOM can reduce the inactivation rate of MS2.EOM can consume additional Fe???,which can be used to inactivate MS2.???is reduced,but IOM can not only consume part of Fe???,but also can protect MS2 to reduced the rate of MS2inactivation.?4?By studying the aggregation of the two viruses under different pH conditions,it was found that at pH=3,the aggregation of TV increased significantly with time,while no aggregation was found at pH=4.5.So the isopotential point of TV was suspected between 3-4.5.While the rotavirus OSU didn't accumulate significantly at pH=3,so the isopotential point was should be less than 3.In addition,the presence of PAA has no significant effect on the virus aggregation.?5?Under different pH conditions,PAA had no significant effect on the inactivation of OSU,but had a significant effect on TV inactivation.And when the pH increased,the rate of inactivation of TV by PAA increased.Combining the aggregation of OSU and TV under different pH conditions and comparing the inactivation results of the two viruses,the aggregation state of the virus was the main factor to affect the virus inactivation under different pH conditions.And the aggregation of the virus caused the instability of PAA to its inactivation.The different initial PAA concentrations was studied and it had no significant effect on the inactivation rate of the two viruses.