Synthesis Of ZIF-8, Cu-hAB MOF And Their Applications In Drug Delivery

In recent years,the incidence rate of cancer is increasing,and it has become one of the major diseases threatening human health.At present,chemotherapy is the conventional treatment of tumor,which has therapeutic effects effects on primary,metastatic and subclinical metastasis.Most of chemotherapy drugs are not selective,only some of them can reach the tumor focus in the process of drug delivery,and the other of them could be absorbed by normal tissue cells,causing serious adverse reactions.Nanodrug carriers can attach drug molecules to the surface of materials,or wrap them inside the materials,which can change the way of drugs entering organism and their distributions in body,effectively control the release of drugs in tumor target tissue,reduce toxic and side effects of chemotherapy drugs,at the same time,control the concentration of drugs within the effective range and reduce dosing frequency.MOF has become an intelligent controllable nanodrug carrier of great research value due to its adjustable pore channel,large specific surface area and controllable structure and good biodegradability.In chapter 2,ZIF-8 nanoparticles was synthesized by liquid-phase diffusion method.In the process of synthesis,surfactants were added to adjust their microstructure,and ZIF-8 nanodrug carriers with different drug release properties were obtained.We also synthesized original ZIF-8 sample without surfactant to show the effect of surfactants on microstructure of ZIF-8.Compared with original ZIF-8,all ZIF-8 modified by surfactants have higher specific surface area and larger total pore volume.In addition,modified ZIF-8 has a good degradation effect in PBS 7.4 buffer solution,which is beneficial to the physiological metabolism of drug carrier.Loading and release performance of surfactants modified ZIF-8 to DOX in PBS buffer solutionshowed,modified ZIF-8 has a good loading effect on DOX.Compared with original ZIF-8,modified ZIF-8 has better drug controlled release performance.Surfactants modified ZIF-8 has no obvious cytotoxicity,but ZIF-8/DOX nanodrug has a significant killing effect on cells.After ZIF-8/DOX is endocytosed by cells,it can release DOX to kill cancer cells in response to pH stimulation.In chapter 3,We synthesized two-dimensional Cu-HAB MOF nanosheets with high drug loading performance,and added anionic surfactant citric acid(CA)to adjust the surface property of Cu-HAB.Cu-HAB(CA)was obtained.Cu-HAB and Cu-HAB(CA)are lamellar structure formed by the accumulation of many fineparticles,and copper ions in Cu-HAB and Cu-HAB(CA)exist in the form of +1 and+2.DOX is still used as drug model in this chapter to explore the loading and release performance of Cu-HAB and Cu-HAB(CA)to DOX.Cu-HAB and Cu-HAB(CA)have very high loading rate to DOX,and Cu-HAB has stronger loading capacity for DOX,which can reach 1047%.In addition,Cu-HAB and Cu-HAB(CA)have excellent controlled release property for the loaded drugs.In simulated acid environment of cancer cells and normal tissue cell environment in vitro,the DOX release rate shows great difference.Cu-HAB and Cu-HAB(CA)nanodrugs have excellent pH stimulation response.