The Preparation Of Copper-based Nanomaterials And Its Application In Photothermal Therapy

Photothermal therapy(PTT)is a new type of minimally invasive treatment for cancer.It can effectively avoid some defects of traditional cancer treatment methods,such as high toxic side effect,big trauma,postoperative cancer spread,etc.Among many photothermal treatment reagents,copper-based nanomaterials have attracted widespread attention for their high photothermal conversion efficiency,low price,and simple synthetic method.In this thesis,two kinds of copper-based nanomaterials have been prepared to reduce the cytotoxicity and improve the photothermal conversion efficiency,the details are as follows:1.Fabrication of multifunctional CuS nanoclusters for chemo-photothermal therapy of cancerA multifunctional CuS nanocluster with controllable drug release,drug tracing and chemo-photothermal therapy is prepared for the chemo-photothermal therapy of cancer.CuS nanoclusters were synthesized using dendrimer PAMAM as a template,with an average particle size of about 6.0 nm and a photothermal conversion efficiency of 30.1%.By coating with hyaluronic acid,the cell uptake was significantly increased,and the cytotoxicity of CuS nanoclusters was effectively reduced.When the material concentration was increased to 300μg/mL,its cell viability was still above 85%.Rhodamine B is attached to the surface of CuS nanoclusters based on covalent bonds,which facilitates drug tracking.Doxorubicin hydrochloride is connected to the surface of CuS nanoclusters through hydrazone bond,which can be controllably released in the acidic environment of cancer cells,and realize the combined treatment of PTT and chemotherapy,killing 63.9%of MCF-7 cells.2.Preparation of Au-Cu2O nanoflowers and their applications in photothermal therapyAn Au-Cu2O core-shell nanocomposite with a flower-like structure was prepared,which effectively improved the photothermal conversion efficiency of the material.The folded structure in the flower-like structure was used to improve the utilization efficiency of the incident light.At the same time,the energy resonance transfer of the Au nano-flower core and the Cu2O shell layer further improved the photothermal conversion efficiency.Based on the synergy of these two factors,the photothermal conversion efficiency of Au-Cu2O nano-flower increased to 51.87%.The photothermal conversion efficiency was higher than most reported copper-based nanomaterials.It is a photothermal therapeutic agent with good application potentials.