Preparation And Bioactivities Of Soluble Dietary Fiber From Young Huskless Barley Leaves

Huskless barley(Hordeum vulgare Linn.var.nudum Hook.f.),known as Qingke in Chinese,belongs to the genus Hordeum of family Gramineae but differs from other conventional barley varieties as it has hull-less caryopsis.Five main huskless barley cultivars were selected and cultivated in laboratory to obtain young huskless barley leaves(HBL).The protein content,amino acid content,total phenolic content,total flavonoid content,chlorophyll content,mineral elements(K,Ca,Mg,Se,Fe,Cu,Zn,Cr),vitamin E content and dietary fiber content were determined.The insoluble dietary fiber content of Qinghaihuang was the highest(47.38%).The soluble dietary fiber from the leaves of Qinghaihuang(HBLSDF)was prepared by xylanase and cellulase combined enzymatic hydrolysis,and the process was optimized.The optimum conditions were as follows: xylanase 995 U/g,cellulase 392 U/g and enzymatic hydrolysis time 2.4 h.Under these conditions,the actual yield of was 22.83%.Component analysis,structure analysis and bioactivity analysis of HBLSDF were carried out.The total sugar content of HBLSDF was 75.23%.Analysis of monosaccharide composition showed that the monosaccharide composition of HBLSDF was 4 monosaccharide components: arabinose,xylose,glucose and galactose(1.24:1.24:3.16:1).The antioxidant capacity analysis showed that HBLSDF owned certain scavenging ability to DPPH·,hydroxyl radical and oxygen radical.In vitro Bile acid-binding capacity experiment suggested that HBSDF had strong Bile acid-binding capacity,which indicated that it had the potential to reduce serum cholesterol.This study provided a theoretical basis for the development of functional food related to young huskless barley leaves.