Morphological Engineering And Fermentation Process Optimization Of Deep Sea Fungi Against Aflatoxin

With the Development of Blue Ocean Strategy in China,more and more scholars are focusing their attention on the study of the ocean.Apparently,there is relatively little research in the deep-sea,however,the metabolites of deep-sea fungi have powerful functions and have good performance in anti-fungal activities.The study on the prevention and control of aflatoxin has always been an important part of biological control,to investigate the inhibitory activity of deep-sea fungi on aflatoxin,the morphological study on 25 strains of culturable filamentous fungi isolated from deep-sea sediments of the South Atlantic Ocean was carried out in this paper,the function of anti-aflatoxin and the effect of two kinds of micro-particles(Alumina and Talc)on the fermentation of two deep-sea fungi(MHE1 and 16-11T)were studied.The fermentation of a deep-sea fungus MHE1 was optimized by multiple factors.In this study,the colony morphology of 25 deep-sea fungi in the South Atlantic was described in detail by fungal plate culture observation method,the micro-morphology of the strain was described in detail and preliminary identification by fungal intercalation culture and the observation and measurement of multifunctional photograph microscope.25 strains of deep-sea fungi belong to 4 genera,namely Penicillium,Aspergillus,Cladosporium,Alternaria.In terms of anti-aflatoxin activity,the samples of 50 fermentation broth of 25 deep-sea fungi were detected by Tip-culture method and some strains with better inhibition activity were obtained,such as: 2-1T,MIE2-1,MHE2,MHE4-1green,16-7T,16-11 T,MHE1,MIE14.The anti-mycelium rate of strain MIE2-1 was up to 94.14%,the anti-aflatoxin rate of strain MIE14 was 100%.There were 9 samples with anti-mycelium rate exceeding 80%,and 20 samples with anti-aflatoxin rate of over 90%.The fermentation optimization of alumina and talc combined rotational speed of 2 fungi was carried out by using Data Processing System.The effects of microparticles on fungal fermentation were discussed,and the optimal fermentation scheme of strain 16-11 T and MHE1 was obtained,and the inhibition of aflatoxin activity of two deep-sea fungi in their optimal scheme was improved,all of which achieved the goal of adding microparticle fermentation optimization.The comprehensive fermentation optimization of multi-factor mixing level of strain MHE1 was carried out,and combined with the application of microparticles,4 fermentation optimization schemes with anti-aflatoxin characteristics were determined.This study shows that deep-sea fungi have great potential in inhibiting aflatoxin.