Study On The Artificial Deep Fermentation Of Cordyceps Sinensis Fungal Powder

Cordyceps sinensis is a traditional Chinese herbal medicine.Cordyceps sinensis by clavicipitaceae insect genus Hirsutella sinensis parasitic Hepialus armoricanus larvae in Alpine Meadow Soils.Cordyceps polysaccharide,Cordyceps acid and cordycepin is the main active substance of Cordyceps sinensis.But due to the less wild Cordyceps and nature,and the price is very high,the current bias in factory production by fermentation.After many years of clinical use showed that the pharmacological effects of Cordyceps products and natural Cordyceps is almost the same,some useful indicators even higher than that of natural Cordyceps sinensis,but its price to far lower than that of the natural Cordyceps.Therefore by Cordyceps sinensis in submerged fermentation obtained mycelium and metabolite very practical value.In this paper,natural Cordyceps isolated strains of bacteria,in the production process of Cordyceps deep liquid fermentation,by changing the fermentation to investigate the physiological environment in different conditions,conditions of mycelia morphology,structure,specific trends and quality indexes of performance parameters,to provide a theoretical reference for the in production process optimization.Focus on the research medium various carbon and nitrogen sources on mycelial growth process in the different effects.The effects of different manufacturers,fish peptone,meat peptone,yeast extract,glucose,enzymatic liquefaction on Yield and quality in production.Fermentation formula in the C/N ratio on production yield and quality is closely linked.To increase the carbon source can improve product mannitol synthesis,but can not guarantee the production yield stability.Based on the optimization of the medium,of mycelial growth environment of experimental optimization.The decisive factors of the fermentation broth pH did not affect the content of mannitol,but may effects to adenosine bacteria powder,residue and other quality indicators;fermentation period such as control in about nine days,can effectively prevent mannitol decreased,and as far as possible,to increase the yield,improve the tank pressure and not very good increase the mannitol content and on mycelial dry weight increase is not very stable.Focuses on the liquid fermentation mycelia growth in the process of feeding mode,a disinfect form and fill the number of material will effect on the yield and quality.Production fed with glucose consumer process control,yield was significantly increased by 15%,and to refine the control of fermentation Cordyceps fungi powder color.Fermentation batch feeding on Yield improvement of some help,but to in the fermentation,post synthesis of mannitol to ensure effective support demand,in order not to affect the quality.