Study On Rosa Roxburghii Tratt Oral Liquid And Its Immune Activity

Rosa roxburghii Tratt is a characteristic resource of Guizhou,which has extremely high nutritional value and broad development prospects.This article studies the immune activity of Rosa roxburghii Tratt and is compounded with selected excipients to develop an enhanced immune system.Natural plant oral liquid with obvious power effect.Provide theoretical basis for the development and application of high-quality prickly pear products,and promote the sound development of Guizhou prickly pear industry.The main contents are as follows:?1?Research on immune activity of Rosa roxburghii Tratt juiceTo study the immune activity of Rosa roxburghii Tratt juice,the high?concentrated 80%?,medium?original juice?,and low?diluted 80%?dose groups of Rosa roxburghii Tratt juice were subjected to cellular immunity?mouse ear swelling method?and humoral immunity?Serum hemolysin method?,antioxidant experiment.Cellular immune indicators include thymus index,spleen index,ear swelling degree,humoral immune indicators include thymus index,spleen index,serum hemolysin value,antioxidant indicators include superoxide dismutase?SOD?activity,glutathione peroxidase?GSH-Px?activity and malondialdehyde?MDA?.The experimental results show that:each dose group has better immunity-enhancing properties,and the Rosa roxburghii Tratt medium dose group has the best effect.?2?Selection of excipients for Rosa roxburghii Tratt oral liquid and optimization of preparation and extractionWith Ganoderma lucidum,Dendrobium nobile and Shiitake mushrooms as auxiliary materials,the three auxiliary materials were extracted in a water bath at an extraction temperature of 70°C,an extraction time of 1.5 h,and a material-to-liquid ratio(g.mL^-1)of 1:30 to prepare the corresponding extracts.Carry out cellular immunity experiment,humoral immunity experiment,and antioxidant experiment.The results showed that there were extremely significant differences between each group and the blank group?P<0.01?,but the Ganoderma lucidum group had the most obvious effect.At the same time,the preparation of Ganoderma lucidum extract and the optimization of the process technology were carried out.The best extraction conditions were:extraction temperature The temperature was 91°C,the extraction time was 4.2 h,and the liquid-to-material ratio was 1:47(g.mL^-1).?3?Optimization of formula design and evaluation of immune activity of Rosa roxburghii Tratt oral liquidUsing the D-optimal mixture design software,a total of 7 different ratios of prickly pear and Ganoderma lucidum were obtained.In vivo experiments in mice?cellular immunity,humoral immunity,anti-oxidation experiments,anti-tumor suppression and C-myc gene expression experiments?Screen the optimal ratio.The results showed that the overall performance of the N3 group was better,that is,the ratio of Rosa roxburghii Tratt juice to Ganoderma lucidum extract was 72.5:27.5.?4?Study on storage stability of Rosa roxburghii Tratt oral liquidThrough the light experiment,temperature experiment and acceleration experiment on Rosa roxburghii Tratt oral liquid,some physical and chemical indicators are observed and measured.The results show that light and temperature can accelerate the appearance,pH value,relative density,total acid,and soluble solids of Rosa roxburghii Tratt It is small,but it has a great impact on V_C and color changes.With the extension of storage time,the color of oral liquid changes from light yellow to light brown.It is recommended to use brown bottle filling and low temperature storage.TheRosa roxburghii Tratt oral liquid products stored for 3 months were tested,and the total number of colonies,Escherichia coli,and mold did not exceed the standard,which met the national standards.