Parameter Improvement Of Intoxicating Degree Detection Model And Mechanism Of Low Intoxicating Degree Of Trace Components

With the improvement of people’s living standards,the requirements for quality of life are also correspondingly improved.For drinking,people no longer only pursue taste,but also health.The concept of drunkenness conforms to people’s requirements,and puts forward an accurate and feasible method to detect the degree of drunkenness or comfort of different liquors,which provides a basis for the production of healthy liquors.In this paper,through analysis of the deficiencies in the original liquor intoxication detection model,optimization and improvement of the liquor intoxication detection model,and the use of the model to study the main fusel,acid,ester ratio of liquor on the intoxication,but also to study the methyl donor betaine and folic acid on the intoxication regulation,and analysis of its impact on ethanol metabolizing enzymes and acute alcoholic liver injury,for healthy white The production and application of wine provide theoretical basis.Through in-depth analysis of the shortcomings of the original liquor intoxication detection model,on the basis of the original liquor intoxication detection model,we optimize it from two aspects.The first is the optimization of blood alcohol parameters.The selection of blood alcohol concentration at the original two time points is changed to the collection of eight time points in four hours,and the metabolic parameters are calculated by the pharmacokinetic software,The parameters Cmax and AUC are used to replace the original parameters;the other is the optimization of behavioral parameters.The water maze experiment is used to replace the previous drum experiment and swimming experiment,which not only keeps the original measurement of its behavior ability,but also increases the measurement of its memory ability.Fusel has a great influence on ethanol metabolism and behavior memory ability,especially when the content of isoamyl alcohol is too high,it will inhibit ethanol metabolism in vivo,mainly because it can inhibit the activity of alcohol dehydrogenase(ADH)and aldehyde dehydrogenase(ALDH),make Cmax and AUC larger than other experimental groups,and reduce the memory ability of mice,significantly extend the latency escape time,so it should be controlled as much as possible Fusel oil content,especially high molecular weight fusel.In the experiment,acetic acid has a better effect on promoting ethanol metabolism,preventing the inhibition of other components on the activity of alcohol dehydrogenase and aldehyde dehydrogenase.Cmax and AUC are significantly smaller than those of other experimental groups,and it can stimulate the memory ability of mice,significantly reduce the latency escape time,and appropriately increase its content in liquor.Betaine and folic acid can accelerate the metabolism of ethanol,which can significantly reduce Cmax and AUC,and show a dose-dependent relationship.ADH,ALDH and catalase(CAT)in the liver of mice after drinking were significantly reduced,but betaine and folic acid could effectively reverse the decrease of ADH,ALDH and CAT activity caused by alcohol,and betaine increased the ADH and ALDH activity more significantly than folic acid.After drinking,the liver injury was serious,the antioxidative ability was decreased,the MDA level of liver was increased,the activities of SOD and GSH-Px were decreased,and betaine and folic acid could protect the liver from alcohol induced liver injury by reducing the MDA level of liver and increasing the activities of SOD and GSH-Px.The results can be used to guide the production of healthy liquor and have a good application prospect.