Curcumin Isolation And Preparation Of Nanoparticles And Its Repairing Effect On Fatigue Stress Injury

It is a normal physiological phenomenon that the body feels tired.However,in a long-term situation of heavy emergency relief work and great physical and mental stress,fatigue cannot be quickly alleviated or eliminated,which may lead to stress response and lead to endocrine system disorders,mitochondrial dysfunction and immune system diseases.Therefore,the search for natural,safe and efficient anti-fatigue and stress active substances have been a research hotspot at home and abroad.From natural extracted from curcuma zingiberaceae plants to curcumin as the research object,the curcumin extract,purification process parameter optimization,the desolvation method is adopted to design build new protein polysaccharide-nano delivery carrier,and by establishing the animal models of fatigue,the research of curcumin and its composite nanoparticles fatigue stress damage repair function in mice.The separation and purification process of curcumin was optimized by column chromatography.Firstly,13 kinds of solid media were screened,and ADS-7 was obtained as curcumin primary purified macroporous resin.Secondly,a single-factor experiment was conducted to determine the optimal loading concentration of 1.5 mg/m L,loading amount of 20 m L,and ethanol concentration of 80%.Then,Box-Behnken design was used to optimize the response surface process parameters of curcumin,and the optimal sample loading was 19 m L.The sample concentration was 1.80 mg/m L.The concentration of eluent was 80%,and the purity of curcumin was 27.93%.Finally,using HPD-300 macroporous resin as the secondary separation medium,the final purity of curcumin was 56.25%.Desolvation method was used to design build new protein and polysaccharide nanoparticles delivery carrier.For the preparation of process parameter optimization of physical stability and in vitro antioxidant activity and release rate of the study results show that the extract zein-gum Arabic-flaxseed gum as a carrier for the transfer of load of curcumin.The optimum preparation parameters were the mass ratio of gum Arabic to flaxseed gum: 8:2.The initial polysaccharide concentration was 0.04%,and the mass ratio of curcumin and zein was 1:40.Curcumin composite nanoparticles have good stability when the ionic strength was less than 100 m M,the p H value was 3 ? 9 and stored for a long time.Curcumin,which was embeddedhad strong antioxidant ability and can be released slowly in simulated intestinal fluid.The fatigue model of mice was established,and the fatigue exercise time and biochemical indexes were measured to comprehensively evaluate the repair effect of curcumin and curcumin combined nanoparticles on fatigue stress injury of mice.The results showed that curcumin nanoparticles could effectively prolong the exhaustion swimming time and exhaustion running time of mice compared with the model group(P < 0.001).The content of serum creatine kinase in the CUR-ZGF nanoparticle dose group(medium and high)decreased significantly,and the content of lactate dehydrogenase increased.This study fully reflected that curcumin combined with nanoparticles had the function of repairing fatigue stress injury,and its anti-fatigue stress activity was better than free curcumin and the mixture of curcumin and wall material,and had certain reference significance for the development of new natural products of anti-fatigue stress.