Application Of Protein-Induced Copper/Manganese Biomimetic Mineralization Nanomaterials In Biosensing

With the development of society,the quantitative and qualitative analysis of disease markers has become the focus of scientific research.The detection of disease markers is commonly used in nanomaterials,among which the application of biomimetic mineralization nanomaterials in biosensors is the main research topic in this paper.Biomimetic mineralization mainly uses biomolecules such as proteins,peptides and DNA to have a high affinity for metal ions through coordination of their amide groups.It maintains the activity of proteins through chemical precipitation and regulation of biomineralization and has certain characteristics of metal salts.This method is not only environmentally friendly,mild and easy to operate,but also has good biocompatibility and chemical stability,and has been widely used in drug delivery,medical diagnosis and sensing analysis.In this paper,metal ions induced by proteins are used to synthesize biomimetic mineralized nanomaterials and their applications in sensors such as electrochemistry,colorimetry and photothermal.1.In this work,a gentle biomimetic mineralization method was used to prepare copper phosphate organic-inorganic hybrid nanoflowers?BSA-Ab2-Cu3?PO4?2 NF?which were used to construct enzyme-free electrochemical immunosensors.The BSA-Ab2-Cu3?PO4?2 NF has the dual nature of integrating biological recognition and signal amplification.The signal enhancement is mainly due to the rich phosphate ion in BSA-Ab₂-Cu₃?PO₄?₂ NF,which can produce a large amount of electrochemical active phosphomolybdate through the reaction with molybdate to generate redox current signal.The electrochemical immunoassay selected positive working potential?+0.14 V?,which can effectively avoid the interference of dissolved oxygen in the electrochemical measurement process.The method we designed showed a wide detection range and a low detection limit in detecting C-reactive protein,with good robustness and potential clinical feasibility.2.In this work,a colorimetric sensor based on the dual enzyme characteristics of glucose oxidase induced mineralization of manganese tetroxide hybrid nanosheets?GOx-Mn3O4 NSs?was proposed for the ultra-sensitive detection of glucose.GOx-Mn3O4 NSs was prepared by green and simple biomimetic mineralization strategy.While maintaining the activity of glucose oxidase,the excellent oxidase-like activity of two-dimensional nanocomposites can directly oxidize the color substrate 3,3',5,5'-tetramethylbenzidine?TMB?.In the detection of glucose,the product H₂O₂ converted under the catalytic decomposition of glucose oxidase can induce the decomposition of Mn3O4,leading to the catalytic activity of TMB decreased with the decrease of the absorbance signal of the solution.and the absorbance of the oxidation state TMB?oxTMB?decreased.Therefore,we have proposed a glucose colorimetric detection method based on multi-cascade enzyme reaction,which provides a new idea for blood glucose analysis and detection.3.From the previous experiments,it can be seen that Mn3O4 nanosheets can directly oxidize the chromogenic substrate TMB into the blue.In this work,we used the biomimetic mineralization method to synthesize the UOD-Mn3O4 nanosheets with oxidase-like and uricase oxidase?UOD?properties,and the nanosheets have also been explored to have good photothermal properties,which can be used to detect uric acid using colorimetric photothermal bimodality.UOD-Mn3O4 NSs can catalyze the decomposition of uric acid to produce H₂O₂ and allantoin.H₂O₂ further reduces the UOD-Mn₃O₄ NSs to Mn²⁺,which causes the loss of oxidation simulation enzymes and photothermal properties,resulting in a decrease in the absorbance signal and the temperature.Therefore,we realized the colorimetric photothermal dual-mode determination of uric acid.