Preparation And Properties Of Polymer Fluorescent Probes Based On Polysiloxane

As a detection method,fluorescent analysis technique has attracted the attention of researchers at home and abroad due to its convenience,good targeting and high sensitivity.As a novel fluorescent probe,polymer fluorescence probes have become an indispensable tool in the process of biological imaging and daily detection.The polymer fluorescent probes have high stability of fluorescence intensity and are easy to form film.Moreover,it can improve the solubility of small molecule fluorescent probe and substrate,increase the possibility of developing composite signal probe,and expand the application field of fluorescent probes.Polysiloxane has attracted wide attention due to its low surface tension,viscosity temperature coefficient,good air permeability,biocompatibility,high and low temperature resistance.Because of these characteristics,polysiloxane as an important material has encountered great opportunities in the field of biomedical engineering.Polysiloxane has developed rapidly in the fields of prosthetic dentistry,tissue engineering,cell growth,wound skin treatment,drug delivery and diagnostic compounds.There are empty 3d orbitals in the outermost layer of the silicon atom,and the 3d orbitals are partially overlapped with the conjugated system,which makes polysiloxane have low LUMO energy level,which is favorable for electron injection.Polysiloxane has been used in many fields of human life due to its sensitivity to chemical modification and its ability to produce short,long or complex polymer particles.By introducing modifying groups into the main chain or side chain of polysiloxane,the special properties of polysiloxane can be given,and the polymer fluorescent probes can be designed to meet different needs.In this paper,polysiloxane polymer fluorescent probes with different detection directions were designed and synthesized from the following three aspects,especially to explore their spectral properties and biological applications.1.A new polysiloxane fluorescent probe V1 was developed with thiomethyl modified naphthalimide as fluorescent group,which can detect thiocyanate in diverse solution specifically.The fluorescence spectrum showed that the probe showed excellent fluorescence stability both in fetal bovine serum solution and in HeLa cells.The stability of fluorescence intensity was compared with the corresponding small molecular model for the first time.Moreover,the probe could also be used in zebrafish and made into test paper to detect thiocyanate in water samples.This provides a theoretical basis for the development of polymer fluorescent probes based on polysiloxane in the future.2.A new fluorescent probe RB-1 was designed by combining the traditional small molecule fluorescent group rhoda mine-B with polysiloxane,which could quantitatively detect metal alu minum ions.Spectral experiments showed that RB-1 had a specific and quantitative detection effect on alu minum ions,and could be detected in solution,cells,and zebrafish in vivo,showing its potential application in biological direction.3.Using naphthalimide modified by triphenyla mine as fluorescent group,a polysiloxane fluorescent probe TR-1 was developed,which could locate lipid droplets.It had the characteristics of aggregation induced lu minescence(AIE).The results showed higher polarity in the solvent,the fluorescence spectrum would red shift and the fluorescence intensity would decrease.Moreover,the probe had the characteristics of AIE,and fluorescence quenching would not occur in the aggregation state.Because there are obvious differences in the number and polarity of lipid droplets between cancer cells and normal cells,the probe could be used to locate the function of lipid droplets and distinguish normal cells from cancer cells.