(+)-Catechin And EGCG On Alcohol-induced Hepatic Lipid Metabolism Disorder

Catechin,widely found in fruits,vegetables,tea,wine and cocoa products,has.strong antioxidant activity and biological effects such as bactericidal,anti-inflammatory and so on.Catechins have five main monomeric components:free-state catechins(C),epigallocatechin(EC),epigallocatechin(EGC),and esterified epicatechin gallate(ECG)and epigallocatechin gallate(EGCG).This paper selected(+)-Catechin and EGCG to study HepG2 cells and Kunming mice to study the effect of Catechin monomer on lipid metabolism.(i)HepG2 cells were used as experimental subjects to detect their lipid metabolites and lipid metabolism genes.Firstly,the concentration and time of alcohol action were determined,and the concentration of(+)-Catechin and EGCG action was determined.At the experiment,indexes such as triglyceride(TG)content,ALT,AST activity,and SREBP-1,PPAR,CPT1,DGAT2 mRNA genes expression level were detected.(1)Alcohol action concentration of 100 mM and action time of 24 h were determined by MTT method;(+)-Catechin interacts with EGCG at cell concentration of 300 mg/L.(2)Triglyceride(TG)content,ALT and AST activity were detected,oil red O was photographed in HepG2 cells:TG,AST and ALT activities were not significantly affected by addition of(+)-Catechin and EGCG compared with those of control group.However,it can effectively improve the lipid metabolism disorder of HepG2 cells induced by alcohol.(3)SREBP-1,PPAR?,CPT1 and DGAT2 mRNA genes were selected for detection:Compared with the control group,the expression level of SREBP-1 mRNA in cells after the action of(+)-Catechin and EGCG was significantly decreased,while other genes were not significantly changed.The expression levels of SREBP-1 and CPT1 mRNA genes were increased after ethanol induction,while PPAR ? and DGAT2 mRNA genes were decreased.The expression levels of SREBP-1 and CPT1 mRNA in alcohol-induced cells under the action of(+)-Catechin and EGCG were lower than those under the action of alcohol group,while the expressions of PPAR ?and DGAT2 mRNA were increased.(ii)Kunming mice were used to establish alcoholic fatty liver model mice.The liver index,serum index,liver H&E and oil red O staining were observed after the model was established successfully.The the formal experiment grouped Kunming mice:Control group,(+)-Cat group and EGCG group.ETOH group and ETOH-EGCG group were established in alcoholic fatty liver model mice.ETOH group mice were alcoholic fatty liver model mice.After the successful establishment of the model,gavage(+)-catechin and EGCG were divided into ETOH-CAT and ETOH-EGCG groups.(1)Detection of serum TG?TC in serum of mice:It was found that the serum TG in EGCG group was significantly lower than that in control group.Compared with mice of ETOH group,the levels of TG and TC in serum of mice of ETOH-CAT group and ETOH-EGCG group were significantly reduced.(2)Compared with the control group,SOD activity in Cat group was higher and there was significant difference.SOD activity in ETOH group was lower than the mice of control group,while MDA content was higher than that in control group.The SOD activity of mice in ETOH-Cat group and ETOH-EGCG group was higher than that in ETOH group,and the MDA content was lower than that in the group.(3)There was no significant change in AST?ALT activity between gavage mice of(+)-Cat and EGCG serum compared with control group.The ALT and AST activity of ETOH group was higher than that of control group,but the ALT and AST activity decreased after gavage.(4)H&E staining showed slight edema in the liver of mice in the control group,normal structure of liver tissues in the mice of(+)-Cat group and EGCG group.No excess lipid droplets were observed in the oil red O staining of the mice of(+)-Cat group and EGCG group.Compared with the control group,the ETOH group showed obvious oil droplets and obvious moderate edema in liver H&E staining,indicating that the liver condition was worse.In addition,oil red O staining showed lipid droplet accumulation and hepatocyte steatosis.Compared with the ETOH group,the liver of mice in the ETOH-CAT group and the ETOH-EGCG group were significantly reduced by H&E staining,and the edema was also improved.Oil red O staining showed that red lipid droplets could be seen in the ETOH-CAT group and the ETOH-EGCG group.But compared with ETOH group,the color became lighter obviously.In this study,HepG2 cells were used in vitro to detect the lipid metabolism indicators and lipid metabolism genes in cells without alcohol induction or alcohol induction.Then,mice were used as experimental objects to detect the serum lipid metabolism indicators and observe the liver morphology.The results showed that the effects of(+)-Catechin and EGCG on lipid metabolism were less under normal conditions,and the effects were more obvious under abnormal lipid metabolism in vivo or in vitro.