Construction Of Ni/Mn Based Metal Oxides Nanoarrays And Their Application In Glucose Biosensors

In this dissertation,nickel oxide nanoarrays decorated with different manganese oxide on the surface of Ni foam were fabricated and investigated systematically.Cross-linking method by using glutaraldehyde?GLA?and bovine serum albumin?BSA?was utilized to immobilize glucose oxidase?GOx?,glucose biosensors based on different substrates were investigated systematically.The main contents and results are as follows:NiO/Mn₂O₃ hybrid nanoflake arrays were directly prepared on the surface of Ni foam by hydrothermal method and annealing process,and glucose biosensor was constructed by cross-linking method.The morphology of NiO/Mn₂O₃ hybrid nanoflake arrays were tuned by optimizing the hydrothermal reaction temperature and reaction time,ammonium fluoride concentration,urea concentration and manganese nitrate concentration.Glucose biosensor based on NiO/Mn₂O₃ hybrid nanoflake arrays achieved high sensitivity(167.0?A�mM^-1�cm^-2),wide detection range?10-2860?mol/L?and good anti-interference ability.Mn₃O₄ nanoparticle decorated NiO nanoflake arrays were synthesized by two-step hydrothermal method followed by calcination treatment.Manganese nitrate concentration,hydrothermal reaction time and reaction temperature in second hydrothermal process were optimized based on the detection of enzymatically catalyzed intermediate H₂O₂.Subsequently,glucose bisoensors were constructed based on optimized substrate and GLA concentration,BSA concentration,drying time and GOx concentration were further optimized to improve the electrochemical performance of as-prepared biosensors.It was found that the sensitivity of as-obtained glucose biosensor was as high as 226.3?A�mM^-1�cm^-2,the detection limit was1?mol/L,and the detection range was 9.9-3665.0?mol/L.MnO₂ was grown on Ni?OH?₂ nanoflake arrays by immersion method,and then annealed in the air to get NiO/MnO₂ hybrid nanoflake arrays.NiO/MnO₂ hybrid nanoflake arrays with different catalytic effects were obtained by adjusting the times of immersion.The glucose oxidase was immobilied by GLA and BSA onto the surface of NiO/MnO₂ hybrid nanoflake arrays to fabricate glucose biosensors.The results showed that the sensitivity of glucose biosensor based on NiO/MnO₂ hybrid nanoflake arrays towards glucose detection was up to 239.4?A�mM^-1�cm^-2.Meanwhile,it had good anti-interference performance.