Technological Research And Engineering Design Of Euryale Ferox Rice Milk Lactic Acid Beverage

Around biotechnology,food processing and development from color,aroma,taste,shape,nutrition,safety and other aspects is an important research direction in the field of food science and engineering.In this paper,the product development and engineering design of euryale ferox rice milk lactic acid beverage was carried out by using the compound raw materials of euryale ferox and rice through amylase hydrolysis,yeast and lactobacillus fermentation,product blending and many more,and a relatively complete product technical route from research to development was completed.The main research is as follows.(1)Enzymatic hydrolysis of raw materials:In order to prevent starch retrogradation after gelatinization of raw materials,improve the silky and delicate of beverages and reduce the roughness of cereal beverages,enzymatic hydrolysis of composite raw materials is required.Liquefaction and saccharification tests were carried out on the raw materials in turn by medium temperature resistant a-amylase and glucoamylase.Taking the content of reducing sugar in the sample as an index,the time required to reach a certain degree of enzymolysis was comprehensively considered,and finally the optimal technological parameters for rice and euryale ferox were determined.Liquefaction parameters were enzyme dosage 10 U/mL,calcium chloride dosage 0.2%,pH6.5 and temperature 95℃;The saccharification parameters were enzyme dosage 10U/mL,pH5.0,temperature 60℃,time 120 min.(2)Screening aroma-producing yeasts and research on fermentation technology:Based on the flavor and ester production from the initially isolated aroma-producing yeast,Z8,Z14 and D1 were optimized and screened as experimental strains for compound fermentation,and the compound ratio is determined to be 5:3:2.Finally,the total solid content,fermentation temperature,inoculum size and fermentation time were taken as variable factors,and the ester yield was taken as index.Combined with single factor experimental results,orthogonal optimization was carried out to obtain the optimized fermentation parameters:total solid content 14%,culture temperature 28℃,inoculum size 3.5%,and fermentation time 2.5 days.(3)Selection of lactic acid bacteria and its fermentation process:Three sterilized lactic acid bacteria yeast fermentation broth were selected as substrates for fermentation experiments.According to the acidity,viable count and sensory characteristics of the beverage,Lactobacillus acidophilus and Streptococcus thermophilus were finally selected as test strains.The ratio of Lactobacillus acidophilus to Streptococcus thermophilus was determined to be 1:3 through experiments of different ratios.When fermented for 24 hours,its acidity reached 0.28(g/100mL).In the fermentation process test of compound lactic acid bacteria,orthogonal optimization was carried out in combination with single factor experimental results,and the best fermentation parameters were determined.The fermentation temperature is 40℃,the fermentation time is 24h,the inoculation amount is 5%,the addition amount of hydrolyzed protein is 0.15%,the fermentation acidity is0.275(g/100mL),the reducing sugar content is 1.87(g/100mL).(4)Beverage blending and product quality index.The control material ratio is 1:10 and the solid content is 9-11%.Adjust the sweet-sour ratio of the beverage product with 3%preserved fruit syrup.Xanthan gum,gellan gum,AGAR AGAR and sucrose ester were selected as the composite stabilizer,and the additive amounts were 0.1%,0.15%,0.035%and 0.20%,respectively.The product quality standard of euryale ferox rice milk lactic acid beverage was established by combining the experiment and referring to the national grain beverage standard,which provided the technical basis for industrial development.(5)Mi Lu Beverage Engineering Design:The process design for the 6000T of the euryale ferox rice milk lactic acid beverage has been completed,the process certification and determination have been completed,and the production technical requirements have been elaborated in detail.Combined with the technological process,the material balance and energy balance were made,the design of typical equipment is completed,and other equipment is calculated and selected.On this basis,the workshop layout was further completed,and the fermenter equipment diagram,workshop layout diagram and process flow diagram were drawn.Finally,the labor capacity and the estimation of water,electricity and steam consumption provided an engineering basis for industrial development.