| In this paper,a variety of pharmacological effects such as anti-tumor,anti –liver,anti-inflammation were studied.To study the extraction technology,and to explore the influence of the antibacterial activity of the extracts of trifoliate cymbidium.The extract was purified and dried,and then the moisture absorption was taken as the index to screen the prescription excipients,select the optimal proportion,study its prescription,obtain the optimal prescription,and further study its quality standard and quality stability.The main research results are as follows:1.Orthogonal design experiment was used to study the extraction process,and the extraction process was investigated and verified.Comprehensive score according to extract rate and amount of extract as an indicator,and by orthogonal experiment,the optimal extract ethanol extract three amt flavonoids content is high,the best extraction technology,optimize the conclusion want to die "soak 30 min,alcohol 60%,2 times,first add 14 times the amount of solvent,extracting time 1 h,the second time and 12 times the amount of solvent,extracting 40 min." The optimized extraction process was verified.The process of solid-liquid separation and concentration was investigated.With filtrate clarity and filtration speed as the indexes,the mesh number of different sieve mesh was investigated,and the mesh number of sieve mesh was determined to be 200 mesh after considering the filtration effect and filtration efficiency comprehensively.According to the actual production experience and the intuitive analysis of the concentration state under different relative density,the optimal concentration condition was determined as "temperature 60 ~ 80℃,vacuum degree-0.075 ~-0.085 Mpa,concentration to relative density 1.10 ~ 1.15(65℃)".The drying process of sanyeqing concentrate can be directly spray dried.Combined with the single factor investigation results of the relative density,inlet air temperature and outlet air temperature of the extract,the final spray drying conditions were determined as: relative density 1.10 ~ 1.15,inlet air temperature 140 ~ 160℃ and outlet air temperature 85 ~ 90℃.2.Bacterial colony counting method was used to determine the bacteriostatic effect of s.epidermidis extract.The results showed that the drugs in the range of 300 mg·l-1 to 1200 mg· l-1 had obvious inhibitory effect on s.epidermidis,indicating that trilobite could significantly enhance the inhibitory ability on s.epidermidis.3.Preparation of extract powder and its powder study.Extract powder was prepared by vacuum drying and spray drying,and its physical properties were evaluated.With water content and powder yield as comprehensive evaluation indexes,the vacuum drying temperature was determined to be 70℃ through single factor investigation.By single factor and orthogonal design test,the optimum spray drying process conditions were determined as air inlet temperature 80℃,liquid inlet speed10%,and the relative density of the liquid phase 1.03.The properties of the two kinds of concrete powders showed that the spray drying powder was uniform powder,spherical or almost spherical in microscopic morphology,and the vacuum drying powder was irregular block.The spray drying powder had lower water content,better fluidity,shorter drying time and higher dissolution rate than the vacuum drying powder.4.The purification process was compared with different purification methods to determine the purification method of macroporous resin,and the process parameters were tested.The purification process was determined as follows: concentrate the concentrate to 1.05 relative density,centrifuge(3000r/min),dilute the centrifuge solution with water to 0.25g/ml,remove impurities by ab-8 macroporous resin column with 1:6 diameter-to-height ratio,add 1BV water to remove impurities,and elute it with 4bv50% alcohol.5.Prescription selection of capsule after physical mixing of extract powder and excipients evenly,the anti-hygroscopic property was investigated,and the superior anti-hygroscopic excipients were preliminarily selected and the hygroscopic law of the drug-auxiliary ratio was investigated to determine the best type and proportion of excipients.In order to maintain moisture absorption effect in the form of appearance,better control of extract powder,hygroscopic,with the aid of experimental software Design-Expert,the optimal mixture Design method,the D-in appearance as examining index,select the best prescription,and the optimal prescription are verified the experimental value and predicted value difference is small,the method optimization result is more accurate and reliable.6.According to the results of accelerated stability investigation,all indicators of this product in the accelerated test within 6 months under the conditions of clinical drug packaging meet the requirements,indicating that the preparation is stable and the tentative validity of this product is 12 months. |
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