Investigation On The Structure And Activity Of Alkaloids In Flueggea Virosa

Through phytochemistry,spectral analysis,computational chemistry and other means,through various separation techniques,such as positive phase separation,inverse phase separation,HPLC,modern spectral methods,and single crystal x-diffraction,21 compounds were separated and identified from the two plants,respectively,four of them come from V.africana,17 from Flueggea virosa in xishuangbanna in yunnan province.Two new compounds,Fluevirines E and Fluevirines F,were isolated from Flueggea virosa.In order to search for the lead compound with good medicinal value,we tested the activity of two new alkaloids.In this experiment,four compounds were isolated from V.africana,one of which was a monoterpene alkaloid and three dimers of alkaloids.one of which was a monoterpene alkaloid and three dimeric alkaloids.V.africana(Apocynaceae)is a tropical tree used as a traditional medicine.Literature report that found three cannabinoid CB1 receptor antagonists from V.africana root bark,which are the fifirst examples of natural alkaloids having cannabinoid CB1 receptor antagonistic activity.We also reported that iboga-type alkaloids isolated from V.africana inhibit capsaicin-induced contraction in the mouse rectum possibly via inhibition of a transient receptor potential vanilloid type1(TRPV1)-mediated pathway.Through the separation experience of the previous plant,I am more familiar with the separation methods and techniques in the plant.In turn,I isolated 17 compounds from the white rice trees collected from Xishuangbanna,Yunnan Province,including2 new alkaloids and 15 alkaloids.It contains 13 monomers,3 dimers and a trimer.It has been reported in the literature that Terpenoids from Flueggea virosa have been detected as a Securinega oligomer alkaloid containing anti-HCV activity and anti-HIV activity.We tested the cytotoxicity of compounds 20 and 21 against five human cancer cell lines HL-60,A-549,SMMC-7721,MCF-7 and SW-480 using the MTT assay,with cisplatin as a positive control.Compound 20 showed cell inhibitionwith an average of 13.61%,4.04%,23.98%,10.53%,and 11.57%.At a concentration of 40 μM,the average values of 21 were 4.65%,22.23%,38.82%,41.59%,and39.81%,respectively.The results showed that both compounds were inactive.In addition,acetylcholinesterase(AChE)inhibitory activity of compounds 20 and 21 was also evaluated using tacrine as a positive control.At a concentration of 50 μM,the percent inhibition of 20 and 21 was 17.95% and 1.97%,respectively,indicating that both compounds did not exhibit AChE inhibitory activity.Due to the low content of the two alkaloids isolated in this experiment,it is not enough to conduct more activity tests.Therefore,it is not known whether the two novel alkaloids have good activity in other aspects,such as anti-tumor,anti-inflammatory and antibacterial,etc.,and it is still necessary for future generations to separate the above two plants and enrich them to a sufficient amount to excavate and explore them.Other aspects of activity.Moreover,this is just a plant,and it can be seen that the natural product discipline is a discipline that needs to be constantly explored and discovered.