Studies On Capparis Spinosa Gels

Objective The extraction process of Capparis Spinosa was optimized.The gels of Capparis Spinosa was prepared.Quality control,in vitro transdermal test,stability test and skin irritation test were carried out to lay a foundation for pharmaceutical research of Cappris Spinosa gels.Methods?1?The methods for the determination of rutin was established by high-performance liquid chromatography.?2?Taking the yield of rutin as the evaluation index,the single factors,such as ethanol concentration,liquid-solid ratio,and extraction times were used to carry out L₉?3⁴?orthogonal test to optimize the extract technology of Capparis Spinosa.?3?On the basis of the single factors investigation,L9?3⁴?orthogonal experiments was carried out with ethanol concentration,precipitation time and temperature as factors to optimize the purification process of Capparis Spinosa extract;?4?Preliminary sceening by single factor investigation,The orthogonal design was used to detect preparation for technical parameters;?5?The quality of the gels of Capparis Spinosa were studied by investigating the quality,cold and heat properties,pH effect,humidity influence,stability tests and skin irritation test;?6?On the basis of establishing the final prescription formulation technology and quality and safety of the gels,the effects of different microscopes on the transdermal effect of Capparis Spinosa gels in vitro were investigated and compared.Results?1?The content determination method of rutin in the Capparis Spinosa was established by HPLC.The linear equation of rutin was y=14.109x-6.9533,R²=0.9999,and the sample size and peak area was showed good linearity in the range of 5.0-100.0?g/mL and had good recovery;?2?Taking the extraction rate of rutin as the evaluation index,the optimal extraction process of Capparis spinosa were set as:the extraction solvent was 17 times of 65%ethanol,the extraction time was 60 min;?3?The optimal purification process were set as follows:the concentration of 30%ethanol was placed at 20?for 12 h,and the filtrate was obtained by filtration;?4?The carpolmer,methylparaben and ethylparaben were put to dossolve by water,then glycerin and extraction were put tagether and stir well,then triethnolamine was added slowly,finally,add azone and stir well to get the sample;?5?The characters,the cold and heat properties,pH value impact and elementary quality test were investigated,which showed that the product quslity was stable;?6?Capparis Spinosa gels of transdermal performance in vitro was good,the micro needle couold effectively increase the transdermal effect of Capparis Spinosa gels in vitro.Conclusions The HPLC method for the determination of rutin which in the Capparis Spinosa and Capparis Spinosa gels was established,which was simple to operate;the extraction and purification process of Capparis Spinosa had strong maneuverability;The formulation process of Capparis Spinosa gels was reasonable,the quality was stable and the safety was high,the transdermal performance in vitrowas good,which can provide experimental research for the development of Capparis Spinosa gels.