Preparation Of Amphiphilic Polymer Nanomaterials And Application In Renal Ischemic Injury And Tumor Therapy

Amphiphilic polymers can self-assemble into core-shell structure.On the one hand,hydrophobic drugs can be encapsulated inside to maintain its stability.On the other hand,the external hydrophilic shell can reduce adsorption of proteins and play an invisible role in vivo to prolong its retention time.Therefore,drug nanocarriers based on amphiphilic polymers have significant advantages in drug delivery and disease treatment.Renal ischemia-reperfusion injury(IRI)is a serious threat to people’s life and health.If IRI can not be effectively controlled,it will lead to severe renal failure.Therefore,it is urgent to find an effective treatment to reduce the incidence and mortality of renal IRI.Meanwhile,cancer is another major disease.Traditional chemotherapy has some drawbacks,such as poor water solubility,high toxicity and easy to produce drug resistance.In recent years,photothermal therapy has attracted great attention.Photothermal therapy using gold nanorods can not completely kill tumor cells,and CTAB on the surface of gold nanorods is highly toxic.Therefore,searching for a low toxicity and effective photothermal therapy agent and realizing the combination of chemotherapy and photothermal therapy is the main direction of attack.Based on the current research status and combined with the advantages of amphiphilic polymer nanoparticles,we constructed nanocarriers with different properties and functions by self-assembly of amphiphilic polymers,and investigated their applications in renal IRI and cancer treatment.The main research results of this paper are as follows:1.Pluronic nanoparticles(PNPs)were prepared by membrane hydration using amphiphilic Pluronic copolymers as carriers.The target ligand folic acid(FA)was modified on the surface of PNPs and the therapeutic drug triptolide(TP)was encapsulated to form TP-FPNPs.It was found that TP-FPNPs exhibited uniform spherical morphology and good stability.At the same time,it also has high drug loading efficiency and can achieve sustained drug release.Because of the targeting ligand of FA on its surface,it has high cell uptake efficicy and superior kidney targeting.In addition,TP-FPNPs have a good therapeutic effect on renal IRI and can significantly reduce the toxicity and side effects of TP.2.Using amphiphilic block methoxy polyethylene glycol-polylactic acid as carrier,the nanocomposite was prepared by double emulsification method.The photothermic agent Ag-Au-Ag NRs and chemotherapy drug doxorubicin(DOX)was encaplusated in nanocomposite to form P-DOX-NRs.The results showed that P-DOX-NRs had good biocompatibility,high stability in vitro,accelerated DOX release under acidic conditions and excellent photothermal conversion performance.At the same time,it can achieve the goal of synergistic inhibition of cancer cell growth by chemotherapy and photothermal therapy,and it is expected to effectively realize the synergistic effect of chemotherapy and photothermal therapy on cancer.