Design,Synthesis,Characterization And Anticancer Activity Of 5-Fluorouracil-1-Silver Acetate Complexes

5-Fluorouracil（5-FU）is an anti-metabolic drug,which destroys the synthesis of DNA and RNA and leads to cell death.It is widely used to treat various tumor diseases.The purpose of this study was to design a silver complex with anti-cancer and anti-bacterial properties.5-fluorouracil 1-acetic acid（5-FUAA）was used as the active ligand,and triphenylphosphine was used as the synergistic ligand.Two5-FUAA-silver（I）complexes were synthesized by reacting with silver salts.The interaction between the complexes and DNA was studied by means of electrochemical experiments,ultraviolet visible spectrometry,fluorescence spectrometry,and so on.It can be concluded from the experimental results that the way of action of intermediate and calf thymus DNA is insertion,and the way of combination of complex and calf thymus DNA is electrostatic.The binding ability of intermediate,complex and calf thymus DNA was the best under neutral condition.Through in vitro cell activity experiments,the anti-tumor effect of the synthesized target complex was determined,and the IC₅₀0 value was obtained.The experimental results showed that the complex had good anti-proliferation activity against human cancer cell lines,and its concentration was within the range of micromole level.The results of this study will provide valuable data for the subsequent design and research of new anticancer drugs with 5-fluorouracil silver carboxylate complexes.