Preparation And Properties Of Nisin@PEGylated MoS₂ And Its Composite Film

Transition metal sulfide MoS₂ has been applied extensively in biomedical field due to its great biocompatibility and physicochemical properties.However,the low antibacterial activity of pure MoS₂ limits its application,so antimicrobial peptide nisin was loaded on the surface of MoS₂ to enhance its antibacterial activity.In this experiment,PEGylated MoS₂ was synthesized via hydrothermal method,and the influence factors of the morphology of MoS₂were explored.Escherichia coli and Staphylococcus aureus were used to test the antibacterial activity,and morphology change of bacteria was observed.The load dosage of nisin on the PEGylated MoS₂ could reach 933 mg/g.When the concentration of nisin@PEGylated MoS₂ was 40?g·mL^-1,the inhibition efficiency could reach 50%.Measurements of reactive oxygen species cumulants could illustrate that ROS is the major bacteriostatic mechanism besides the physical damage of MoS₂.Chitosan was used as the substrate to synthesis the nisin@PEGylated MoS₂/chitosan film.When the content of samples reached 1.0%,the films showed the best mechanical and barrier properties.The antibacterial activity of film was originated from the antibacterial activity of chitosan and the release of nisin@PEGylated MoS₂.