| Background and purpose of the study:Bacterial infections are infectious diseases caused by pathogenic bacteria.Globally,bacterial infections have become a serious public health problem,and their impact on both human health and economic development has been dramatic.According to statistics,millions of people worldwide die every year due to bacterial infections,including common diseases such as pneumonia,tuberculosis,diarrhea,and sexually transmitted diseases;in addition,bacterial infections are a common cause of inflammatory response triggered by the body,and inflammatory response is the body’s first line of defense against infections,but excessive or inappropriate inflammatory response may cause damage to the body;therefore,proper control of inflammatory response is needed while treating bacterial infections to facilitate the clearance of infection and wound healing.Before the advent of antibiotics,many means of treating bacterial infections were very limited,and with the widespread use of antibiotics,they were once considered a hope for solving the problem of bacterial infections;however,with the increasing frequency of antibiotic use,many bacteria have developed resistance,resulting in some conventional antibiotics no longer being effective in treating infections;in addition,for various reasons,including misuse of antibiotics,inhospital infection control In addition,the emergence of multi-drug resistant bacteria has become a global public health problem due to various reasons,including misuse of antibiotics and poor infection control in hospitals.Therefore,the search for new antibacterial and anti-inflammatory strategies and treatments has become increasingly important,and the use of nanocomposites and other methods to address this challenge has become a hot research topic.The application and research of gold and silver nanomaterials are growing,and the related research in the field of antibacterial and anti-inflammatory is particularly prominent,where gold(Gold,Au)nanomaterials can produce high temperature sterilization by photothermal therapy(PTT),while silver(Silver,Ag)nanomaterials are classical antibacterial agents;some studies have confirmed that gold-silver(Au-Ag)bimetallic nanomaterials contribute to the effect of PTT,but because the structural stability of branched Au-Ag bimetallic nanomaterials is poor and thus affects their effect,therefore,nanocomposites with a core-shell structure can be formed by wrapping polymers and other materials on their surfaces,and the polymers with good biocompatibility are mainly polydopamine(PDA),polyethylene glycol,etc.),polyethylene glycol,etc.PDA can modify inorganic materials by simulating mussel adhesion,and it has been demonstrated that PDA also has photothermal effects.However,with the depth of research,some nanomaterials have a single antibacterial mechanism,the effect is not obvious and other problems become more and more serious,therefore,on the basis of gold nanoparticles and PDA photothermal antibacterial,silver nanoparticles sterilization of this experiment added antibacterial nanomaterials with simulated enzymatic activity at the same time Molybdenum disulfide(MoS2),and the antibacterial effect of composite materials and anti-inflammatory potential of the composites were investigated.Research methods:In this study,a novel MoS2-based composite nanomaterial was prepared by wrapping Au-Ag bimetallic nanoparticles with PDA and then loading them on the surface of MoS2 nanosheets.The composite nanomaterials were characterized by scanning electron microscopy,transmission electron microscopy,atomic force microscopy,ultraviolet absorption spectroscopy and Fourier transform infrared spectroscopy;the anti-staphylococcal ability of the composite nanoparticles was investigated by plate counting method and scanning electron microscopy;in terms of anti-inflammation,cellular level experiments were performed to briefly evaluate the anti-inflammatory effect of the composite nanoparticles and to monitor the amount of reactive oxygen species.Finally,the antibacterial and anti-inflammatory ability of the materials was verified again by a mouse skin infection defect model.Results of the study:The results showed that the prepared composite nanomaterials were clearly branched,with uniform particle size,uniformly wrapped with PDA shells on the surface and well dispersed,and successfully loaded on MoS2 nanosheets;the prepared composite nanomaterials had a high inhibitory effect on Staphylococcus aureus,and could significantly inhibit its growth,which may be due to the combined Au-Ag bimetal,PDA and MoS2 in the materials.In addition,the composite nanomaterials can also reduce the production of intracellular pro-inflammatory factors and promote the production of anti-inflammatory factors,which may be due to MoS2 is able to exert its anti-inflammatory effect by scavenging reactive oxygen species to regulate immunity.Conclusion:In conclusion,the composite nanomaterials obtained from this study,which were loaded with MoS2 after PDA wrapped with Au-Ag bimetal,have good antibacterial and anti-inflammatory effects,which provides a certain reference for the development of new antibacterial and anti-inflammatory drugs.However,the safety and effectiveness of the composite nanomaterials still need to be evaluated more comprehensively... |
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