The Preparation Of Molecularly Imprinted Polymers Containing Pyrene And Their Applications In Analyses

Molecular imprinting technology(MIT)is widely used in various fields because of its predictability and specificity.Traditional molecularly imprinted polymers,due to the lack of signal transduction,can only be applied by using complex detection methods such as low resolution mass spectrometry,liquid chromatography-mass spectrometry and so on.In this work,molecular imprinting technology and fluorescence spectrometry are combined to prepare a fluorescent molecularly imprinted polymer,which were applied for rapid and quantitative detection of the targets.Then,two kinds of fluorescent molecularly imprinted polymers were designed and synthesized using pyrene-containing derivatives as fluorescent signal probes.The selective recognition performance and the analytical testing of decabromodiphenyl ether(DBDPE)and hemoglobin(HGB)were studied.The main work of this paper is as follows:1.A novel fluorescent molecularly imprinted polymer,DBDPE-FMIP,was synthesized with pyrene methyl methacrylate as a functional monomer and DBDPE as template molecule.The DBDPE-FMIP was then applied to the detection of DBDPE.In this work,the parameters of elution time and adsorption time were optimized,and the performance of DBDPE-FMIP was characterized by fluorescence spectroscopy.The results showed that the DBDPE-FMIP has long fluorescence lifetime,excellent photostability and high sensing ability,displaying a linear response to DBDPE in a concentration range of 0.5-30μM with a detection limit of 0,061 μM.Meanwhile,the DBDPE-FMIP exhibited obvious recognition specificity for DBDPE over its analogues.The standard spiking method was used to test the real water samples.The results showed that the recoveries were 99.0-104.3%and the standard deviations were 3.82-4.51%.In conclusion,the study revealed that the amounts of DBDPE in water samples can be detected quickly,sensitively and selectively using the as-prepared DBDPE-FMIP.2.A novel fluorescent molecularly imprinted polymer,HGB-FMIP,was synthesized with pyrenemethanol as functional monomer,HGB as template molecule and nano-silica as carrier.The HGB-FMIP was then applied to the detection of hemoglobin.In this work,the elution time and adsorption time were studied,and the structure and morphology of HGB-FMIP were characterized by means of FTIR,SEM,TEM and fluorescence spectroscopy.The results showed that the HGB-FMIP has a good core-shell structure,and the thickness of the coating is about 18.15 nm.Meanwhile,the results of fluorescence spectrometry showed that the linear range of HGB-FMIP to HGB was 0-0.1 mg/mL and the minimum detection limit was 0.003 mg/mL.In addition,the developed HGB-FMIP was used to detect the actual samples.The results showed that the recovery ratio was 99.7-108.3%with RSD of 3.01-5.82%.In summary,the study illustrated that the as-prepared HGB-FMIP can be used to detect biological macromolecular hemoglobin rapidly,sensitively and selectively.