Recongnition Property Of Molecularly Imprinted Thiamphenicol And Bovine Hemoglobin On Silica Gel Surface

Molecular imprinting polymer is a kind of fuctional polymer materials,which possess theability of molecular recognition and develops in recent years. This kind of polymer can beused as selective catalyst, solid phase extractant and sensing detector of the chemical sensor,which have a unique function in the field of medical and environmental, solid phaseextraction, biosimulation sensor and so on. At the basic of summerizing the basic theory ofMolecular imprinting technology, preparation technology and its progeress in appliedresearch, this paper used the surface molecular imprinting method and producted printedpolymers,then discussed the performace of these polymers. The conent as shown follows:(1) Thiamphenicol as a template elements, Methacrylic acid as functional monomer,EDMA as the cross linker, AIBN as the initiator and by the imprinting technology of surfacegrafted molecular to produce the imprinted polymerson the surface of silicon surface. Thendiscussed the performance of this polymer using the static equilibrium and HPLC．Thisimprinted polymer was applied as the stationary phase of high-performance liquidchromatography,as a results, a mixture of structural analogues and a template elements can beseparated baseline by this way. Compared with non-imprinted polymer, this imprintedpolymer have a better adsorpted effection to the sulphone mycin.(2) Using the silica gel modified with aldehyde as a carrier material, bovine hemoglobinas template molecular, acrylamide as the fuctional monomer, N,N’-methylene bisacrylamideas the crosslinker and then by the method of surface molecular imprinting technique toproduce the polymer which a unique recognition ability to the bovine hemoglobin. Discussedthe influence of the elution rate to the imprinting effect of the polymer, evaluation thepolymer by the selective adsorption and Scatchard curve. The results show that molecularlyimprinted polymer have a good adsorption capacity and specific identification to the bovinehemoglobin.（3）Using the silica gel as carrier to produce the molecularly imprinted polymers andevaluation the polymer by the equilibrium absoption and competition experiment. The resultsshowed that the imprinted polymers had a high adsorption efficiency and high selectivity.