Effect Of Soybean Dietary Fiber On Blood Glucose And Pancreatic Damage In Diabetic Rats And On Ovarian Senescence

In this study,the bean dregs which produced after soybean processing were used as experimental material,and the process of extracting dietary fiber from soybean dregs by complex enzyme method was optimized by response surface design.The hypoglycemic effect of soybean dietary fiber was explored by using type 2 diabetes model rats as experimental subjects.The hypoglycemic effect and the mechanism of soybean dietary fiber and its repairing effect on pancreatic oxidative damage caused by type 2 diabetes mellitus were explored.The female aging model rats were selected as experimental subjects,to explore the delayed action and mechanism of soybean dietary fiber on ovarian aging in rats.This study will provide a theoretical basis for the study of soybean dietary fiber in reducing blood sugar,repairing oxidative damage in the body and delaying the aging process of the ovaries.The main findings are as follows:(1)In the experiment of optimizing the enzymatic extraction of soy dietary fiber in bean dregs by response surface design,the optimal process conditions for extracting soybean dietary fiber by complex enzyme method were determined by response surface analysis combined with actual values.The α-amylase addition amount 0.4%,The enzymatic hydrolysis time ofα-amylase was 25 min,the amount of neutral protease was 0.04%,the time of neutral protease hydrolysis was 30 min,and the yield of dietary fiber reached 72.43%.(2)In the experiment of soybean dietary fiber on hypoglycemic in diabetic model rats,compared with the model group,the weight of soybean dietary fiber in the type 2 diabetic rats was significantly higher than that in the model group(P<0.01).The fasting blood glucose level of the rats were significantly lower than that of the model group(P<0.05).The serum GSP content of rats were significantly decreased(P<0.01),and the contents of INS and GLY were significantly higher than those in the model group(P<0.01).Pancreatic histopathological sections showed that the pancreatic tissue of rats in each group were damaged.The degree were significantly reduced,the number of islet cells were increased,and the degree of cell edema deformation were reduced.(3)In the experiment of soybean dietary fiber on oxidative damage repair in diabetic model rats,the ratio of pancreas and the weight of the medium and high dose soybean dietary fiber group decreased significantly(P<0.05).The contents of serum GSH-Px and SOD in ratswhich from the medium and high dose soybean dietary fiber groups were increased in different degrees compared with the model group(P<0.01).the MDA levels in serum were decreased in different degrees(P<0.01),and it were observed from pancreatic tissue sections.The degree of pancreatic tissue damage in rats were significantly reduced.The number of islet cells increased,and the degree of cell edema deformation was alleviated.(4)In the experiment of anti-aging effect of soybean dietary fiber on rat ovary,the FSH and LH content of rats in serum in each dose group were significantly lower than those in the model group(P<0.05).The serum E2 and AMH contents in the middle and high dose soybean dietary fiber groups were significantly higher than those in the model group(P<0.05).The results of ovarian pathology showed that the degree of aging damage of ovarian tissue in each group were significantly reduced.