Tumor vaccine,as a prophylactic approach,has a broad application prospects in tumor immunotherapy.It has been a main target that delivering tumor associated antigen(TAA)into antigen processing approach and activation of antigen-specific cytotoxic T cells.But activating T cells response effectively usually requires a relatively long interval of immunization.In this therapy pathway,initiating adaptive immune responses rapidly are critical,so it can be a critical step to shorten this interval.Antigen-presenting cells(APCs)are the initiators of ingesting,processing and presenting antigens,as the main target of messenger ribonucleic acids(mRNA)vaccines.In vitro transcribed mRNA which express specific proteins is a more promising therapeutic and vaccine approach,not only with shorter production cycles,but also avoids many of the safety issues associated with viruses in DNA-based systems,such as integration into the genome.Based on the significant advantages of mRNA,mRNA vaccines have shown potential as activators of adaptive immunity.However,mRNA is so highly susceptible to nucleases and is difficult to enter cells directly,that delivering mRNA vaccines into dendritic cells(DCs)systematically is hampered by various technical challenges.Nanoparticle combine with mRNA can remedy these disadvantages.A series of nanocarriers that deliver mRNA have thus been developed.Here,we investigate cationic lipid-assisted nanoparticles(CLAN)as a delivery vehicle for mRNA vaccines.We found that CLAN encapsulating mRNA encoding tumor-mode antigen(TAA)can effectively stimulate the maturation of DCs and promote the activation and proliferation of antigen-specific cytotoxic T cells,both in vitro and in vivo.Intravenous injection of CLAN encoding ovalbumin(OVA)mRNA in mice can activate OVA-specific T cell responses in vivo and effectively slow down tumor growth in the invasive E.G7-OVA lymphoma model.In general,CLAN is an excellent mRNA vaccine delivery platform that can induce a efficient adaptive immune response.For further optimization,including combination with various adjuvants,the efficacy of vaccine may be increased. |