Study On Rapid Detection Of 2,4,6-trinitrophenol By Fluorescent Probes

2,4,6-trinitrophenol（also known as picric acid,TNP,PA）is a common explosive,whose explosive power is stronger than the 2,4,6-trinitrotoluene（TNT）.In addition,TNP is also widely used in pharmaceutical intermediating,leather manufacturing,gunpowder manufacturing,dyes manufacturing and other industries.In the process,TNP is easily leaked to the environment,contaminating groundwater and soil,which makes it an environmental pollutant.It has received increasing attentions in recent years due to TNP’s powerful explosive capacity and the environmental pollution.For the sake of national stability,people’s social stability and environmental protection,it is of great significance to develop a rapid detection method for TNP.In this dissertation,the detection methods for TNP were analyzed and summarized.A small-molecule fluorescent probe PDI-M with a simple structure was synthesized based on an easy process and used for the rapid detection of TNP.The interaction and sensing mechanism between the fluorescent probe and TNP has been explained;the detection performance was optimized,and a test strip was prepared for simulated sample detection.The main contents of our work are listed as follows:1.A small-molecule fluorescent probe PDI-M was synthesized by a one-step reaction with perylenediimide（PDI）,2-bromoethylamine hydro bromide and imidazole.We analyzed its molecule and structural characteristics by ¹H NMR and HR-MS.2.We studied the fluorescent properties of the fluorescent probe PDI-M.Under the optimized experimental conditions for detection,the fluorescence titration experiments of the probe PDI-M with TNP showed that TNP has a good quenching effect on the probe;the fluorescence intensity of the probe at 547 nm was measured.In the range of 20-80μmol/L,the concentration of TNP showed a good linear relationship with the value of I₅₄₇.The detection limit was calculated.This result is of great significance for the application of probe PDI-M in quantitative detection of TNP.3.We also discussed the visual detection limit,response time,stability and selectivity test of probe PDI-M.Under the 365 nm hand-held UV lamp,we set 20μmol/L as the visual detection limit of PDI-M to TNP,and the probe PDI-M also achieved the visual detection of other interferences;the response time of the probe PDI-M is short（about 1min）and the response signal has good stability;the probe shows high selectivity and good sensitivity toward TNP.At last,the interaction and sensing mechanism of probe PDI-M detecting TNP was explained and discussed.4.We simulated explosive dust by adding TNP to blank dust.The probe was used to detect TNP in experimental dust.This method has good accuracy and precision.The average recovery rate was relatively high and stable.In addition,a test strip based on PDI-M for the detection of TNP was prepared.We also used this test strip to detect the experimental dust samples and achieved good results.