Preparation Of Micro-nanochannel Detection Device And Its Application In Biomolecular Detection

Abnormal changes in the structure or concentration of biomolecules are always the source of mutations and infections.The lack of effective pre-diagnosis is the main reason for the rampant spread of various diseases.In recent decades,with the rapid and continuous development in biotechnology and nanoscience,building biomolecular detection systems based on nanomaterials has attracted extensive attention of scientists in this field.Comparing to the biological channels,the solid-state nanochannels possess the characteristics of mechanical robustness,multifunctional surfaces and flexible and controllable geometric structures with similar scale and dimension to the activity area of biomolecules and biomolecules.In this paper,the biomolecular detection devices with multi-sensitivity and selectivity based on solid-state nanochannels were designed with the following parts:1.The controllablly grafting monodisperse Au NPs into AAO nanochannels was used to realize the effective and controllable recombination of multi-dimensional nanomaterials.Brieflly,we developed a facial method of in situ decorating Au NPs on the inner surfaces of AAO nanochannels by using the dual-functional PDA as adhesion and reduction agent.Simultaneously,the size and the number density of Au NPs can be precisely controlled by adjusting the variable factors of reactions,including PDA concentration,ambient temperature,hydrochloric acid concentration and filtration duration.According to statistics,the particle size of Au NPs varies from 30 to 80 nm,while the number density varies from 109 to 1012 m-2.Moreover,The size and number density of decorated Au NPs would affect the total surface area and volume,which controlled the steric hindrance of AAO nanochannels by regulating the grafting amount of DNA into their inner surfaces.The AAO membrane with high Au NPs size and number density showed a higher steric hindrance than the lower one.2.The effective detective system for glucose and other biomolecules was built based on conical nanochannels,which were prepared by heavy ion bombarding and one-way chemical etching a polyethylene glycol terephthalate(PET)membrane.The detection molecules of DNA,glucose and PSA protein were decorated onto the small pores of the resulted conical nanochannels by introducing the “bridge” molecules with terminal amino groups firstly.Meanwhile,AIE molecules(TPEDB)was introduced into the nanochannels for optimizing the detection accuracy and specificity of glucose molecules.The above detection system can not only accurately detect DNA(detection limit 1 μM)and glucose(detection limit 1 p M)molecules,but also highlight the positive and negative voltage transmembrane current rectification ratio,which provides a more stable and reliable detection parameter.