Application In Rapid Detection In Food Based On Background Fluorescence Quenching Immunochromatographic Assay

Objective:A quantitative determination card of Aflatoxins B₁,Aflatoxins M₁,Chloramphenicol,Clenbuterol,Salbutamol,Ractopamine was developed by utilizing the background fluorescence quenching immunochromatographic assay?bFQICA?in order to detect the content of Aflatoxins B₁ in the cooking oil,Aflatoxins M₁ and Chloramphenicol in the milk,Clenbuterol,Salbutamol and Ractopamine in pork rapidly.Established a detection method of Linconycin and Kanamycin in the milk.Methods:By utilizing the background fluorescence quenching immunochromatographic assay,based on the activities of Aflatoxins B₁,Aflatoxins M₁,Chloramphenicol,Clenbuterol,Salbutamol,Ractopamine antigen and antibody,the best concentration of Aflatoxins B₁,Aflatoxins M₁,Chloramphenicol,Clenbuterol,Salbutamol,Ractopamine antigen in the T line and antibody in the small cup of the detection system were screened.Methodology validation of Aflatoxins B₁ in the cooking oil,Aflatoxins M₁,Chloramphenicol,Linconycin and Kanamycin in the milk,Clenbuterol,Salbutamol and Ractopamine in pork and sample determination were conducted by this detection system.Results:1.The detection system was composed by the Aflatoxins B₁ antigen concentration of 0.5mg/mL and the Aflatoxins B₁ antibody concentration of 1.0?g/mL.The concentration of Aflatoxins B₁ in this detection system ranged from 1.3 to 50.0ng/mL.The minimum detection limit of1.1ng/mL.2.The detection system was composed by the Aflatoxins M₁ antigen concentration of 0.5mg/mL and the Aflatoxins M₁ antibody concentration of 1.2?g/mL.The concentration of Aflatoxins M₁ in this detection system ranged from 0.0014 to2.0ng/mL.The minimum detection limit of 0.0009ng/mL.3.The detection system was composed by the Chloramphenicol antigen concentration of 0.4mg/mL and the Chloramphenicol antibody concentration of 0.6?g/mL.The concentration of Chloramphenicol in this detection system ranged from 0.001 to 1.0ng/mL.The minimum detection limit of 0.0008ng/mL.4.The concentration of Linconycin in this detection system ranged from 0.102 to 60.0ng/mL.The minimum detection limit of 0.088ng/mL.5.The concentration of Kanamycin in this detection system ranged from 0.2319 to60.0ng/mL.The minimum detection limit of 0.2003ng/mL.6.The detection system was composed by the Clenbuterol antigen concentration of 1.5mg/mL and the Clenbuterol antibody concentration of 1.0?g/mL.The concentration of Clenbuterol in this detection system ranged from 0.019 to 2.0ng/mL.The minimum detection limit of 0.011ng/mL.7.The detection system was composed by the Salbutamol antigen concentration of1.0mg/mL and the Salbutamol antibody concentration of 0.5?g/mL.The concentration of Salbutamol in this detection system ranged from 0.016 to 4.0ng/mL.The minimum detection limit of 0.009ng/mL.8.The detection system was composed by the Ractopamine antigen concentration of 0.5mg/mL and the Ractopamine antibody concentration of1.0?g/mL.The concentration of Ractopamine in this detection system ranged from 0.021to 2.0ng/mL.The minimum detection limit of 0.007ng/mL.The result of T-test showed that there was no significant difference between measured values of sample by bFQICA and by National Standards of the People,s Republic of China?p>0.05?.Conclusion:This method was based on the reaction of antigen and antibody,so was specificity and sensitive.Testing liquid samples do not need sample preparation,testing a sample only 8 to 12min.Solid samples need simple pretreatment affter operation.The methodology of the sample was stored in the qr code.This method would rapid and precise for detecting Aflatoxins B₁in the cooking oil,Aflatoxins M₁,Chloramphenicol,Linconycin and Kanamyci in the milk,Clenbuterol,Salbutamol and Ractopamine in pork.