Study On The Properties Of Single/multi-fluorescent Group Tripod Fluorescence Probe

Anions and cations are widely distributed in nature.Many metal ions exist in human activities and play a crucial role in maintaining life system,such as biological metabolism,osmotic regulation,catalysis and so on.Anions are also involved in a host of living body processes,such as chloride ions,phosphate ions,that are identified and transmitted,as well as in concentration control processes.Therefore,if there is a significant changed in the distribution of these ions in nature,it will have an immeasurable impact on the sustainable development of human society.Some ions are highlytoxic to organisms at very low concentration.Therefore,it is necessary to find accurate,rapid and real-time detection methods is extremely important,and the fluorescence sensor can perform the characteristics due to high sensitivity,good selectivity,good stability,simple and rapid operation,and can perform visualization of imaging points without damage to the samplefor this need.Through the non-covalent interaction in the microscopic field,ions and molecules for specific recognition of analysis,and they are reflected by fluorescence signals,thus realizing in situ real-time monitoring at the molecular level.Fluorescence sensors have great practical value in the detection and identification of biologically relevant ions.Four kinds of tripod fluorescent probes were studied.1)Tripod fluorescent probe1 modified by the same 4-（benzothiazole-2-hydroxy）phenol fluorophore clusters.Study showed that the probe fluorescence enhanced high selectivity and high sensitivity.The detection Cr³⁺,Al³⁺,Zn²⁺can also be detected by ratio fluorescence and ratio absorption F^-,the probe has a ESIPT mechanism,which can by Cr³⁺,Al³⁺,and F^-;2)by 4-（Benzothiazol-2-hydroxy）-phenol fluorophore and a rhodamine fluorophore-modified tripod fluorescent probe 2.study have shown that this is a fluorescent probe based on the fluorescence resonance energy transfer process as a recognition mechanism.The probe detection of Hg²⁺,Al³⁺ions,The fluorescence emission of probe-Hg²⁺,probe-Al³⁺and probe-Zn²⁺complex is sensitive to the polar effect of 1,4-dioxane/H₂O solvent,and can be used as organic reagent in 1,4-dioxane.The indicator of water volume is sensitive,simple,intuitive,and the naked-eye defensible probe can detect the change of water content in the solvent within a certain range through the change of the color and intensity of fluorescence;3)The azobenzene clusters and one rhodamine B clusters modification Tripod probe 3,studies have shown that it is possible to visually detect quantitatively or semi-quantitatively the detection of a wide pH range from acid to strong base;ratiometric colorimetric simultaneous detection of F^-,AcO^-,H₂PO₄^-,fluorescence enhancement and visible absorption Identify Hg²⁺ions.4)The tripod fluorescent probes modified by three fluorescent clusters of Rhodamine B,NBD,and hydroxynaphthalene formaldehyde,showed that the selectivity of Zn²⁺and Hg²⁺could be detected by solvent effect and time resolution.