Study On Secondary Metabolites And Bioactivities Of Aspergillus Terreus XWC21-10 From Porites Pukoensis

Marine microorganisms continue to attract the interest of natural product research as a prolific source of structurally diverse and bioactive natural product,many of which play an eminent role as potential leads in discovery.Coral-derived fungi is an extraordinarily diverse species,which generate many secondary metabolites with unique structure and excellent activity.Therefore,the structural diversity and pharmacological activity of the compounds produced by these microorganisms have also been studied extensively.In this study,we deliberated the antibacterial activity,and antioxidant and enzymatic inhibitory activities of a corel-derived fungi XWC21-10 fermented broth isolated from the coral fungus of Porites Pukoensis(Scleractinia corals)from Xuwen Coral Nature Reserve.The fungi was identified as Aspergillus terreus with morphological and phylogenetic analysis.Studying the fermentation broth and mycelium extract of active ingredients,preliminarily determining the fermentation broth and mycelium extract containing chemical constituents were relatively abundant,and they had similar components.And its free radical scavenging activity of DPPH,acetylcholinesterase inhibitory activity,brine shrimp lethality and anti-inflammatory activity were studied.The results show that DPPH had clear active ingredients at the same time,the activity and inhibition of acetylcholinesterase containing the fermentation broth and mycelia of Artemia,the lethal activity was not obvious,the fermentation broth and mycelium of RAW264.7(murine macrophages)having cytotoxic,anti-inflammatory activity was not obvious Combining fermentation broth and mycelia extract,purification,and ultimately determine the structure of the compound nine,they were butyrolactones I(ZB-4-3),butyrolactones Ⅶ(ZB-4-3),butyrolactones Ⅱ(ZB-5-2),physcion(A-7),(Z,Z)-9,12-Octadecadienoic acid(A-3),Z-9-trans-14-octadecensaeure(A-4),ergosterolperoxide(A-5),emodin(A-1)and asterric acid(ZB-2-1)were obtained.These compounds were screened by antibacterial,scavenging activity of DPPH,acetylcholinesterase model and anti-inflammatory activity.Three kinds of butyrolactoneⅦ,butyrolactones Ⅱ and butyrolactones I had different anti-bacterial,Butyrolactones Ihas anti-bacterial on Vibrio parahaemolyticus and Staphylococcus aureus,MIC was 6.25μM,3.125 μM,respectively.Butyrolactone Ⅶ and butyrolactones Ⅱ have anti-bacterial against large intestine and Staphylococcus aureus and Escherichia coli,with MIC of1.56μM,respectively.Asterric acid and three butyrolactone had anti-DPPH activity,EC50 of 36.68 ± 0.17 μM,24.56 ± 1.21 μM,31.30 ± 0.45 μM and 56.29 ± 0.35μM respectively.Asterric acid also has acetylcholinesterase inhibitory activity.The anti-inflammatory activity of two butyrolactone compounds was studied by Griess method.The release of NO from RAW264.7 cells was studied by LPS.The determination of two butyrolactone compounds had a good anti-inflammatory effect.This study isolated compounds from Porites pukoensis a fermentation product of symbiotic fungi,and the biological activity of the butyrolactone Ⅶ for the first time from the coral isolated and measured its antibacterial,scavenging activity of DPPH radical double.This study enriched the secondary metabolites of epiphytic fungi in the coral reef,which laid the foundation for the study of natural products from the genus coral.