The Design Of Molecular Imprinting Fluorescent Sensors Based On Dual Recgnition Functional Ionic Liquids And Their Applications In The Evironmental High-risk Factors Analysis

With the improvement of living standards and the rapid development of science and technology,industrial production and agricultural production have been booming day by day.Residues of aromatic amines and phenolic endocrine disruptors(PEDs)are greatly harmful to the environment and human health.Studies have shown that aromatic amine pollutants enter the body through the respiratory tract,digestive tract or skin can cause changes in the structure and function of DNA in human cells,carcinogenic,teratogenic and mutagenic effects,leading to blood poisoning,skin eczema and dermatitis Sick and so on.Phenolic endocrine disruptors cause genital disorders in animals and humans,abnormal behavior,decreased fertility,impaired nervous and immune systems,and death or even extinction of juveniles.Therefore,developing a fast,sensitive and effective method of detecting these pests for safety and pollution control is an urgent need to maintain public safety and is crucial for the protection of the environment.This project innovatively synthesized duel recognition alkenyl ionic liquids as fuctional monomer of molecular imprinting sensor materials,which not only output fluorescent signal,but also can be used as cross linker promoting imprinting polymerization.Superior performance of fluorescent analysis platform to detect environmental high-risk detection is contructed based on the excellent selective detection ability of molecular imprinting combined.Using ionic liquids as functional monomer also help to improve the fluorescent intensity benefiting from high charge density of ionic liquids.We finally achieve the environmental high-risk factors such as aromatic amines and phenolic endocrine disruptors p-nitroaniline and 2,4,6-trichlorophenol with highly sensitive and reliable detection.The main work are as follows:(1)With the modified ionic liquid(3-chloro-(3-methylene-9-anthryl)-1-vinyl-1 hydro-imidazole)as the fluorescent functional monomer,p-nitroaniline was used as the template molecule,Ethylene glycol dimethacrylate was used as crosslinking agent,and molecularly imprinted polymers were prepared by bulk polymerization molecular imprinting technique.Fluorescence sensors were successfully constructed.The synthesized novel ionic liquid molecularly imprinted polymers were characterized by means of NMR,LC-MS,FTIR,SEM and particle size analysis.The experimental results show that the synthesized molecularly imprinted polymer has uniform spherical shape,high fluorescence emission intensity,good specificity and high sensitivity,high detection rate(<1 min)and high reusability Recovery of at least four times),the measured p-nitroaniline in the concentration range of 10-M-10μM has a good linear relationship with a linear correlation coefficient of 0.992,detection limit of 9 nM.The comparison between simulated wastewater and high performance liquid chromatography showed that the prepared novel ionic liquid molecularly imprinted polymer could be successfully applied to the determination of p-nitrophenol in environmental water samples.(2)A novel ionic liquid([9,10-bis {(N-vinylimidazolyl)methyl} anthracene with 1-vinylimidazolium ionic liquid modified to high fluorescence intensity by 9,10-bischloromethylanthracene]Chlorine)as fluorescent functional monomer,N,N-methylenebisacrylamide as crosslinking agent and 2,4,6-trichlorophenol as template molecule,and the fluorescent molecularly imprinted polymer.The synthesized novel ionic liquid molecularly imprinted polymers were characterized by means of NMR,LC-MS,FT-IR and SEM.The results showed that the fluorescence intensity of the obtained ionic liquid[9,10-bis {(N-vinylimidazolyl)methyl1｝ anthracene]chloride was 3-chloro-(3-methylene-1-vinyl-1-hydro-imidazolium ionic liquid fluorescence intensity of two times.In addition,the constructed fluorescence sensor has good selectivity and strong anti-interference ability.The measured 2,4,6-trichlorophenol shows a good linear relationship in the range of 1-100 nM with the minimum detection limit of 684 pM,and Successfully applied to the detection of environmental water samples.(3)Synthesis of a modified novel ionic liquid[9,10-bis {(N-vinylimidazolium)methyl} anthracene]chloride was used as the fluorescent functional monomer and N,N-methylenebis Acrylamide as cross-linking agent,2,4,6-trichlorophenolas template molecule and AIBN as initiator.Silica-based ionic liquid surface molecularly imprinted polymer microspheres(SiO2 @ MIPIL)were obtained by surface molecular imprinting.The Si02 @ MIPIL was characterized by NMR,LC/MS,SEM,FTIR,TG,TEM and XRD.The effects of SiO2@ MIPIL fluorescence sensor were investigated by using laser confocal spectroscopy and fluorescence spectrophotometer.Quenching phenomenon.The results show that the sensor has excellent selectivity for 2,4,6-trichlorophenol,fluorescence quenching and 2,4,6-trichlorophenol concentration in the range of 0.1-50 nM showed a good linear relationship,the lowest detection Limit to 89 pM.The prepared fluorescence sensor has the characteristics of responsiveness and fastness,and provides a new method for the detection of 2,4,6-trichlorophenol,which can be successfully applied to the detection of environmental water samples.