Preparation Of Active Substances From Sunflower Plate And Their Hypouricemic Activity

Gout has a long history.It is a kind of ancient "rich people’s disease." In recent years,the number of patients suffering from gout has continued to increase,and there has been a trend toward youth.The current clinical drugs for the treatment of hyperuricemia and gout are very limited and cannot be cured.Western medicine is the majority of therapeutic drugs,but the toxic side effects of western medicine threaten human health.In this context,many studies have turned to natural compounds of plant origin.Sunflower is one of China’s oil crops and its planted area is also very impressive.The seeded sunflower disk,the majority are used as livestock feed.In the book of ancient medicine China on the records of the medicinal value of the sunflower plate can fend off the cold dampness.In the folk prescription,it is also used for the treatment of dysmenorrhea,asthma and so on.So far,there are few reports on the treatment of gout with sunflower disk.In this paper,sunflower plate and traditional Chinese herbal tablets were used as raw materials for the preparation of enzymatic hydrolysates,aqueous extracts and alcohol extracts.The in vitro XO inhibitory activity of the obtained samples was compared.The method used in this paper was to determine the XO inhibition rate by HPLC.The results showed that:In the enzymatic hydrolysate group,raw material was Chinese herbal tablet,.the enzyme hydrolysate of enzyme C had the highest inhibitory rate of XO in vitro,and reached 37.88±1.56% at 20 mg/m L,while in sunflower plate group,the enzyme B enzyme hydrolysate had the best inhibitory activity in vitro,and reached 47.58±1.76% at 20 mg/m LIn the aqueous extract group,the preparation condition of Chinese herbal medicine was two,and the inhibition rate of XO in vitro was the highest.When the concentration is 15 mg/m L the XO inhibition rate reached 62.52 ± 0.013%.In sunflower group,the highest inhibition rate was six,and the XO inhibition rate can reach 84.94±0.69% at 20 mg/m L.In the alcohol extracts prepared under the same conditions,the in vitro XO inhibitory activity of the two groups of Chinese herbal decoction and the sunflower disk showed no significant difference.The inhibitory rate was 42.53±0.72% and 47.49±1.76% at 20 mg/m L.In summary,the highest XO inhibitory activities in vitro of both traditional Chinese medicine pieces and sunflower plates are in the form of aqueous extracts.The better performance of the two water extracts is the WEHA.The antioxidant activity of WEHA was investigated,the results showed that the WEHA exhibited good antioxidant activity toward hydroxyl radical,ABTS radical and DPPH radical at the final concentration of 0.1-1 mg/m L.Under the concentration of 1 mg/m L,the scavening rate can reach more than 75%.The superoxide anion free radicals,at concentrations higher than 2.5 mg/m L,showed a better scavenging effect.In this study,potassium oxazine and hypoxanthine combined use of mouse model of hyperuricemia.Compared with normal blank group(154.49±15.14 μmol/L),uric acid level in serum of model group increased rapidly,reaching 266.39±17.87 μmol/L,and the difference reached a very significant level.The positive drug group(allopurinol 10 mg/kg)significantly lowered the level of serum uric acid,which was 28.06±10.14 μmol/L,while the levels of serum uric acid in low,medium and high doses of WEHA also decreased to 103.62±9.65 and 132.16±12.46,158.49±17.41 μmol/L,close to the normal blank group.The results showed that WEHA could reduce the increase of uric acid in the serum.The determination of serum XO activity showed that the activity of XOD in the serum of WEHA group was also lower than that of the model group,which decreased by 21.91%,20.60%,5.33%,respectively.In the low medium high dose group,the activity of XOD in the liver was also lower than that the model group,which decreased by 7.7%,11.64%,11.46%,respectively.The effect of WEHA on mouse health is also one of the important considerations.First,the body weight of mice before and after comparison can be initially determined,sunflower plate water extract does not affect the health of mice;and urea nitrogen,creatinine levels and organ coefficient of synergy test results also proved that WEHA basically does not cause kidney damage,and even organ protective effect.The study of this experiment has some positive significance for the development of the medicinal value of sunflower plate,and it provides a reference for the screening of gout drugs in the screening of natural compounds.