| As a powerful analytical tool for qualitative and quantitative detection of molecules,surface-enhanced Raman spectroscopy?SERS?can also provide much structure and content information of molecule.SERS is simple,fast,non-destructive,and highly sensitive in terms of analysis and detection.For this reason,its application is very wide in fields,such as biomedicine,art identification,food safety,and public safety.Based on the potential of SERS,it is applied to the illegal addition of foods and drugs.In this paper,SERS was used for detection Rhodamine B in condiments and aromatic compound of phenformin hydrochloride?PHE?.The silver nanowires were prepared through the improved method of polyol.Ag NWs were assembled onto glass slides through self-assembly method.Moreover,in our experiment as-prepared Ag NWs@Glass were used as a SERRS substrate to detect Rhodamine B at the excitation wavelength of 532 nm.Rhodamine B can be adsorbed on the surface of silver nanowires by electrostatic interaction.The SERS substrate was characterized by TEM,SEM and XRD,and the enhancement factor of the substrate was calculated?4.01×106?,which proved that the Ag NWs@Glass substrate has a good SERS enhancement ability.Experimental conditions such as pH value and soaking time on SERRS performance were studied and optimized.Under the optimized conditions,the SERRS intensity at 1648 cm-?1? exhibited a linear relationship with the concentration of Rhodamine B in the range of 1.0×10-9-1.0×10-55 mol L-1 and detection limit?S/N=3?is as low as 0.3 nmol L-1.The corresponding correlation coefficient of the linear equation was 0.996.This method based on Ag NWs@Glass for the detection of Rhodamine B in three kinds of condiment was investigated and the recoveries were 89.0-93.6%,85.0-98.7%,and80.0-94.0%,respectively.The AgNP@?-CD were synthesized by a simple and direct one-step methodology.Since the hydrophobic cavity of?-cyclodextrin traps molecules containing hydrophobic groups,the substrate and the analytes are measured.The mixed droplets of the material are added to the hydrophobically treated silicon wafer to form a highly hot coffee ring for the detection of phenylephrine hydrochloride?PHE?.Furthermore,densely-packed substrate produced a large number of hot-spots to improve the SERS effect.The SERS performance of AgNP@?-CD substrate was characterized by using o-Phenylenediamine dihydrochloride as the probe molecule,the concentration dependence curve of o-phenylenediamine was obtained and the enhancement factor?1.58×106?of AgNP@?-CD substrate was calculated,indicating that the coffee ring substrate has a good enhancement effect.The excitation wavelength and the pH value were optimized.The method displays a linear response for PHE in the 7.0×10-8-1.0×10-6 mol L-11 concentration range,and the limit of detection is as low as 8.0×10-9mol L-1.The method was applied to the detection of PHE in healthcare products and commercial tablets,with recoveries between 95.3-105.0%and 96.3%-105.0%,relative standard deviations between 2.64%-5.16%and 2.87%-4.08%,respectively.It can be seen that the method has a credible and satisfactory result in the detection of PHE in actual samples.In addition,the aspirin,which is an aromatic compound containing a hydrophobic group,has a good detection effect. |
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