Study On Analytical Method For Migration Of 55 Plastic Components In Barley Wine And Yoghourt

Plastic additives refers to the macromolecular polymer dispersion in the plastic structure,not seriously affect the molecular structure of the plastic,can improve the plastic quality or cost reduction,impels improvement of plastic base material of workability,physical,chemical,and other functions,and increase the base material of physical and chemical properties of a few small molecular compounds,these additives play an important role in improving the various performance plastic.Plastic as one of the more and more common food packaging,in the process of contact with the food,inevitably there are all kinds of plastic additives to the food in the migration,thus causing food contamination,form a potential threat to human body health.The alcohol in the barley wine,the lipds and acids in the yogurt are good solvent for many plastics additives,which can facilitate the migration of these small molecules into food.The products such as highland barley wine and yogurt can be contaminated by multi-class and multi-component plastic additives.Therefore,it is great significance to study the methods of high flux analysis of plastic additives in highland barley wine and yogurt.The content and resetual of this paper mainly have the following several aspects.1.A method for simultaneous determination of 55 of plastic additives in highland barley wine by ultra-performance liquid chromatography-tandem mass spectrometry?Ultra-performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS?was developed.Plastic additives including antioxidants,plasticizers,ultraviolet light absorber,colorant,and so on in barley wine were studied.Samples were diluted to 20%alcohol with water,and extracted by acetonitrile and separated with salting out.The analytes were separated on a reversed phase ACQUITY BEH C₁₈8 column?2.1 mm×50 mm,1.7?m?and eluted gradiently with methanol and 0.1%formic acid,and multiple reaction monitoring mode?MRM?was used with electrospray ionization in positive ion mode?ESI⁺?.Analytes were quantified using external standard method.The linear calibration curves were obtained in a wide range with the linear relative coefficients?r²?more than 0.99.Recoveries from 3 spiked levels ranged 71.8%-96.3%and the relative standard deviation?RSDs?were1.6%-14.7%?n=6?.The limits of detection?LODs?and the limits of quantification?LOQs?for the analytes were 0.2-40?g/L and were 0.6-133?g/L.Twelve batches of barley wine samples were determined and a total of 20 plastic components were detected,with levels of 2.7 to 20.8?g/kg,belonging to several classes of compounds.2.A grouping scan method has been developed to determine 55 plastic additives including antioxidant,plasticizer,ultraviolet light absorber,and so on in yoghourt usingultra-performanceliquidchromatography-tandemmassspectrometry?UPLC-MS/MS?.Samples were extracted by acetonitrile and cleaned up with Waters Oasis HLB solid phase extraction column.The UPLC system equipped with a ACQUITY UPLC HSS T3 C₁₈8 analytical column?2.1 mm×50 mm,1.7?m?,utilizing methanol-0.1%formic acid as mobile phase in gradient elution mode.Electrospray ionization ion source at positive ion mode?ESI⁺?for multiple reaction monitor analysis was used,and quantified by external standard method.The calibration curves were good linear in wide area and the correlation coefficient over 0.99.The average recoveries from spiked yogurt at the 3 concentrations ranged from 62.4%to 93.5%with relative standard deviation?RSD%?from 2.4%to 13.4%.The limits of detection?LOD?were 0.2?g/kg⁴0?g/L.Twenty-three plastic additives with concentration7.0³9?g/kg were found in 20 samples.This method can simultaneous identify and quantitative analysis plastic additive compounds in yoghourt.