Function Study Of SsPre1 Of Sclerotinia Sclerotiorum

Sclerotinia sclerotiorum(Lib.)de Bary is a worldwide important plant pathogen that endangers crops and vegetables.It has a wide range of hosts and can cause diseases and yield reduction of many oil crops,causing huge economic losses.Therefore,it is of great significance to explore the pathogenic mechanism of the genes related to Sclerotinia sclerotiorum.In this experiment,Split-marker gene knockout technology was used to construct a gene knockout box containing hygromycin resistance gene hph.PEG-mediated protoplast transformation was used to screen transformants on hygromycin-containing medium.The transformants were purified by increasing hygromycin concentration and verified by PCR.Finally,two mutant strains of ?SsPrel-1 and ?SsPrel-2 were obtained.Then phenotypic observation and pathogenicity study were carried out to clarify the function of SsPrel in Sclerotinia sclerotiorum.The resuLts showed that compared with wild type(WT),SsPrel mutant had slower colony growth rate,less sclerotia formation and less acid production,which indicated that SsPrel had a reguLatory effect on sclerotia mycelium growth,sclerotia formation and acid production.The decreased viruLence of the knockout mutant indicated that SsPrel was involved in the pathogenic process of Sclerotinia sclerotiorum.The development of this experiment is helpfuL to understand the function of SsPrel of Sclerotinia sclerotiorum and provide theoretical basis for the prevention and control of Sclerotinia sclerotiorum.