| The "high temperature filming" technology was developed by Zhang Youjun,a researcher from the vegetable and flower research institute of the Chinese academy of agricultural sciences.This technology can increase the control effect of cockroaches to 100% by using a blue no-drop film with a thickness of 0.10 mm when the light intensity exceeds 55000 Lux without adding any chemicals.Yield.High temperature in order to define the "sun" laminating technology on soil microorganisms and soil physical and chemical properties change,thus promote the mechanism of leek production,the project is in the Chinese academy of agricultural sciences institute of vegetables,flowers experimental base,looks like the selection of soil fertility and leek consistent field,resect leek set after filmed(SF)and control(SC)processing,according to the 5 sampling way to deal with respectively before and after treatment 1 day,5 days,10 days,20 and 40 days of soil sampling,using high-throughput sequencing technologies to detect microbial diversity,metagenomics in soil samples,In addition,the contents of N,P,K and p H in soil samples were analyzed,and the relationship between microbial polymorphism,macro genome information and soil physical and chemical properties before and after treatment was studied.The main research results obtained by Illumina Mi Seq high-throughput sequencing technology and physical and chemical properties analysis are as follows:(1)"High-temperature filming" technology can can reduce the diversity of microbial community in soil.The results of microbial population analysis showed that the dominant bacteria in soil were the same in the high temperature film mulching group and the control group.The microbial population structure of SF1 and SF2 soil samples was significantly different from that of other soil samples.Firmicutes and Clostridia were abundant in SF1 samples.Moreover,the abundance of Gammaproteobacteria and Pseudomonadaceae species in SF2 soil samples was relatively high.(2)It is clear that "High-temperature filming" technology can promote the beneficial microbial population in soil.The abundance of Chloroflexi,Bacillus,Proteobacteria and Firmicutes was significantly increased in SF1 soil samples.(3)According to the measured physicochemical properties of the earth,the content of hydrolytic nitrogen in SF1 samples in the filmed soil increased by 30.69%.Soil organic matter increased by 6.45% in the SF1 sample of the film-treated group,4.16% in the SF2 sample,3.58% in the SF4 sample,and 8.23% in the SF5 sample.The content of available potassium in soil was 20.5% less than that in SF2 sample and control group.For the p H value of soil,the soil covered with film was slightly higher than that of the control group within the range of 7.23-7.67.(4)Combined with the analysis results of soil physical and chemical properties,the "insolation and high temperature mulching" technology treated six physical and chemical factors in soil,namely,hydrolytic nitrogen,total potassium,total phosphorus,rapidly available phosphorus,organic matter and temperature,with a high contribution value to RDA1.Among them,the effects of hydrolytic nitrogen,available phosphorus and temperature on soil microbial population diversity were significantly correlated.(5)The soil microbial population was mainly bacteria(89.13%),followed by archaea(1.45%)after treated with "high-temperature filming" technology through the analysis of macro-genome data.(6)The results were obtained by the macro-genome sequencing of 4 representative soil samples: the abundance of some functional genes in the soil treated with the "high temperature and filming" technology changed,especially the abundance of enzyme genes involved in the nitrogen metabolism pathway and methane metabolism pathway.The abundance of SF1 and SF2 related enzymes in soil samples in the nitrogen metabolism pathway was significantly higher than that in the control group.The relative enzyme abundance in SF1 of soil samples in methane metabolism pathway was higher than that in the control group,while that in SF2 and SF5 samples was lower than that in the control group.(7)SF1 sample comment results in KEGG database function,Metabolism accounted for 76.45%,Genetic Information Processing accounted for 8.52%,Environmental Information Processing accounted for 3.34%,Organismal Systems accounted for 0.67%,Organismal Systems accounted for 0.21%,of which treated with "high temperature filming" technology have an effect on soil function metabolism.(8)Nitrososphaera has a high contribution to the functions of Function 3 AT and Function 4 ABS in the CARD gene pool through annotation of the resistance gene pool.(9)The comparison between SF5 samples and SFQ samples in the control group by means of macronomic analysis showed that there was no significant difference in functional bacteria in this membrane coating technology.In this study,both microbial diversity,macro genome information and soil physical and chemical properties showed that "high temperature filming" technology was conducive to improving the types and number of beneficial microorganisms in soil and promoting the effect of beneficial microorganisms on soil fertility.Finally,its original ecosystem can be restored,and this environment-friendly technology can be promoted sustainably. |
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