| This research in the Antarctic exploration of Marine sediments(Sed1,Sed2,Sed3)and intertidal zone(IT1,IT2)as experimental samples,based on the traditional cultivation methods,Usess the Miseq high-throughput sequencing technology and microbial cultivation method,microbial community of sediment and intertidal zone,and further understanding of microbial diversity,microbial ecology function,the relationship between the three environmental impact factors.Metagenomic technology can further understand the ecological functions of marine microorganisms in the Antarctic marine ecosystem,understand the diversity of functional genes,and understand the potential relationship between microorganisms and the environment on the basis of understanding the structure of the polar marine microbial community.Miseq high-throughput sequencing technology conducted a preliminary study on the microbial diversity and community structure in Sed1,Sed2,Sed3,IT1 and IT2,and explored the abundant microbial resources near the Great Wall bay of Antarctica based on the statistical clustering analysis of phylum,class and genus levels.Bacteria common to the five samples included Bacteroidetes,Proteobacteria,Verrucomicrobia,Actinobacteria and Planctomycetes.The common bacteria are Lutibacter,Gammaproteobacteria,Fusibacter,Maritimimonas and Blastopirelilul.Pseudomonas is the dominant bacterium in Sed1,Sed2 and Sed3,while Granulosicoccus is the dominant bacterium in IT1 and IT2.Chromadorea and Diatomea are dominant classes in intertidal zone and sediment respectively.By constructing the clone libraries of functional genes of sediment samples(Sed1,Sed2,Sed3)and intertidal samples(IT1,IT2),the functional gene types and their species contribution were analyzed by metagenomics.The results showed that: For Metabolism,Poorly characterized,Cellular processes signalingand Information storage signaling-processing,the four major functional contributing genera are Proteobacterial,Bacteroides and Actinobacteria.In the analysis of carbon sequestration bacteria with secondary and tertiary functional genes,more than 40% of the sediments were carbonsequestration bacteria,and the intertidal zone was less than 20%.Impact factor correlation analysis of pH,Zn,Cu in intertidal sample Ilumatobacter,Actinobacteria and Nocardiobe as the main influence factors and pH had a positive effect on IT1 and IT2 in intertidal samples,and a negative effect on Sed1,Sed2 and Sed3 in sediment samples.Using R2 A and 2216 E two configuration by seawater medium of Antarctic Great Wall intertidal samples IT1 and IT2 for the separation and purification of culturable bacteria,isolated 142 strains of bacteria,16 S rRNA genes of isolates for PCR amplification and sequencing and sequence alignment,found that these strains from four phylum,Proteobacteria,Actinobacteria,Bacteroidetesand Firmicutes,distributed in 20 genera,There are 80 proteobacteria,which reached 56.3% of the total isolates.In the second place were 47 bacteroidetes,12 actinomycetes and 3 firmicutes,accounting for33.1%,8.5% and 2.1%.In the isolates of IT1 sample,proteobacteria were more than 70%,distributed in 8 genera.The dominant genera in IT1 are Flavobacterium and Paraglaciecola.Sulfitobacter,Zhongshania,Psychromonas,Oceanisphaera,Gelidibacter,Cellulophaga,Aequorivita,Paeniglutamicibacter,Arthrobacter,Micrococcus,Planococcus is IT1 samples of endemic genera.The Marine microbiota near the Great Wall bay of Antarctica is a complex community composed of many microorganisms and an important representative of the transition from Antarctic terrestrial ecosystem to Marine ecosystem.The related results are of important scientific reference significance for the in-depth understanding of the ecological status of microorganisms in the polar Marine ecosystem,the assessment of the response and feedback of the polar Marine microbial community to global climate change,and the protection of the polar ecological environment. |
PDF Full Text Request