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A Study On The Toxicology Of Different Soil Pollution Sources To The Biology

Posted on:2020-01-18Degree:MasterType:Thesis
Country:ChinaCandidate:G C LiuFull Text:PDF
GTID:2370330599958067Subject:Cell biology
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With the development of industrialization and agricultural production,global soil pollution is becoming more and more serious.Soil pollutants mainly include organic pollutants,inorganic pollutants,biological pollutants.Pesticides and metals have serious effects on soil pollution.Plants and animals that live in the soil will continuously and passively absorb soil pollutants from the soil environment.This paper studies the effects of soil pollutants on plants and animals.The first chapter mainly introduces the pollution status of DDT,Co,Ni.And the influence of these substances on human health.The second chapter mainly probes into the toxicological research of DDT stress.Objective: To explore the effect of DDT on mortality and DNA damage.Methods: Using the comet experiment method,to observe the percentage of the tail length,tail distance and tail DNA of the comet.Through statistical analysis,to get up the dose-effect relationship between DDT stress and mortality.Results:(1)DDT has significant toxic effect on the mortality of the springtails.The Median lethal dose is 51.67,43.41,20.57 and 14.94 ug/L on 24,48,72 and 96 h,respectly.(2)The maximum damage degree can reach to 3 levels.The effects of DDT stress on comet tail length,tail length and DNA content in the tail of springtails cells are significantly related to the dose-effect relationship.Conclusion:There is a positive correlation between the mortality of acanthi and DDT concentration.There is a dose-effect relationship between DDT and DNA damage.The third chapter has two parts.The first part is mainly exploring Co and Ni stress to four different plants(Iris sibirica L.,Acorus calamus L.,Typha orientalis Presl,and Phragmites australis(Cav.)Trin.ex Steud.).In the progress found the tolerance of plants were different heavy on metals.Then the hyperaccumulative plants of heavy metal Co and Ni were screened,and the second part of the study was carried out to repair the soil by using heavy metal-rich plants.Objective: To explore the effects of heavy metals on four plants and explore the enrichment plants and soil restoration of different heavy metals.Methods: Four plants under different concentrations of heavy metals were stressed byhydroponic experiments.The plant height,water absorption,dry matter and the contents of heavy metals in each organ were measured by inductively coupled plasma mass spectrometry(ICP-MS).By SPSS 19.0 for Windows statistical software to correlate and regress analysis,single factor variance analysis,etc.Results:(1)Under the Ni stress,the change trend of Iris sibirica L.biomass was not obvious.The trend of biomass of Typha orientalis Presl is increase.(2)Iris sibirica L.roots absorbed most of the transport to the ground,and Acorus calamus L.root absorption of Ni and Co more than stay in the roots.(3)The ability of Iris sibirica L.to accumulate heavy metals Ni is much higher than that of Acorus calamus L.;the capacity of Iris sibirica L.to accumulate heavy metals Co is much higher than that of Acorus calamus L..Conclusion: Every physiological measure of the four plants changes with the concentration of heavy metals.The superaccumulative plants of Ni are Siberian iris and Typha orientalis Presl.The superaccumulative plants of Co are Siberian iris and Acorus calamus L..
Keywords/Search Tags:soil pollution, DDT, Ni, Co, dose-effect relationship
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