The Reaserch Of Construction Of 3D Neural Network Based On Human Induced Pluripotent Stem Cells

The matured cells are reprogrammed into induced pluripotent stem cells(iPSCs)with exogenous transcription factors,and this kind of stem cells have similar functions and characteristics as embryonic stem cells,the use of iPSCs to construct three-dimensional neural networks has great significance for the study of central nervous system and neurological diseases.Mature adult cells with disease genes are reprogrammed into pluripotent stem cells.Nerve cells derived from such cells have the original characteristics of diseases.Such disease-specific iPSCs provide samples for studying the mechanism and pathogenesis of diseases,and also provide impetus for drug development and treatment plan formulation.Based on three-dimensional scaffolds and human induced pluripotent stem cells,this paper constructs a 3D neural network structure,completes the differentiation of human induced stem cells into motoneurons and the construction of neural networks.The main work and conclusions are as follows:1.hiPSCs were differentiated into neural cells(hiPSCs-NC)by adding induction factors.The inverted cells showed that the differentiated cells exhibited neuronal physiological morphology.Immunofluorescence staining and reverse transcriptionpolymerase chain reaction showed hiPSCs-NC can express its specific proteins and characteristic genes.2.hiPSCs were differentiated into motor neurons by adding induction factors.The differentiated cells were identified by immunofluorescence staining and flow cytometry,respectively.It was proved that hiPSCs were successfully differentiated into motor neurons with high efficiency through the differentiation method explored at the present stage.3.The 3D scaffolds obtained by photolithography and electrostatic spinning were arranged in an orderly way with microcolumn structure,the spinning spacing is less than 8 μm,and the size was smaller than the size of the cells.hiPSCs-NCs was cultured and differentiated on this 3D scaffolds and characterized by confocal laser scanning microscopy and other instruments...