Transcriptome Analysis On Iron Reduction And Extracellular Electron Transfer Of S.oneidensis MR-1

S.oneidensis MR-1 is one the important model electricity producing bacteria with extracellular respiration.It can use electron from organic matter in waste water and transfer the electron to extracellular electron acceptor,such as metal oxides.As a kind of dissimilatory metal-reducing bacteria,it can not only transmit electrons by reducing the external metal oxides,but also use various non-metallic substances,such as carbon electrodes or sulfides as electron acceptors.It has received widespread attention.It has become one of the model strains to study extracellular electron transfer mechanisms.On the other hand,the microbial fuel cell is a form of bioenergy that has been widely studied for a long time.By building a two-chamber microbial fuel cell,we analyzed electrochemical information of S.oneidensis MR-1in two different conditions.In this paper,microbial fuel cell were constructed by using S.oneidensis MR-1 as anode catalyzer in two different environments.Then we analyzed S.oneidensis MR-1in different environments by using electrochemical analysis,transcriptome analysis and protein analysis techniques.Consequently,we found the changes of electrochemical,transcriptomic and protein data.Using sequencing data and multiple analysis methods,the differential expression of genes of the S.oneidensis MR-1 in the metabolic pathway was analyzed.Finally,we isolated proteins from S.oneidensis MR-1 that cultured in different environments.With several databases,we understand the function of some corresponding proteins,and the proteins with high expression levels were investigated and analyzed.