Construction Of Heavy Oil-degrading Bacteria Consortium And Preparation Of Mealy Microbial Agent By Lyophilization

With the increase of heavy oil exploitation and processing,the pollution of coastline caused by heavy oil leakage gradually increased,and the problem of oil pollution coastline caused worldwide attention.Heavy oil is a kind of petroleum hydrocarbon with high content of asphalt and asphaltene.The density and viscosity are large,the composition is complex and the biodegradability is difficult.However,the repair of heavy oil pollution environment,especially microorganism,Pollution and other advantages still have a good research and application value.In this paper,based on the characteristics of heavy oil,the heavy oil degrading bacteria were screened and identified on the basis of the literature research.The construction and evaluation of the complex flora were carried out.From the beneficial preservation and application,the freeze-Technical conditions were studied,and achieved good results.In order to obtain the microbial strain resources with good degradation effect,65strains of bacteria with heavy oil as the sole carbon source were isolated from the heavy oil contaminated soil of Liaohe Oilfield,among which 6 strains A-10,A-17,B-11,B-15,C-1 and C-4 heavy oil degradation efficiency of 20%or more.6 strains were identified as Roseomonas sp.,Rhizobium sp.,Bacillus sp.,Chryseobacterium sp.Dietzia sp.,Enterobacter sp.by physiological and biochemical identification and 16S rDNA respectively;A high-efficient complex flora F46 composed of A-17,B-11,B-15and C-1 has a good thick The degradation rate of heavy oil reached 62.85%under the optimum conditions of initial heavy oil concentration 300 mg?L^-1,pH 8,temperature 30?and salinity 15 g?L^-1.High grade;F46 dry powder preparation was prepared by vacuum freeze-drying technique,and the final compound protective agent formula was combined with glycerol 1.0%,sucrose 5%,sodium glutamate 1.0%,skim milk powder10%?both mass fraction?The dry survival rate reached 82.52%,and the storage stability was better.The degradation rate of the heavy oil was 8%lower than that of the F46 strain after 7 days of the activation of the freeze-dried bacteria.Repair has a good application potential.