Effects Of High Sulfur Coal Covering On Soil Microbial Diversity

Sulfur-oxidizing bacteria in high-sulfur coal can produce acid,and acid infiltration will reduce the pH value of the underlying soil covered by high-sulfur coal,and increase the content of organic nutrients,thus affecting soil microbial diversity.Taking Wuda Coal Mine as an example,the effects of high sulfur coal cover on soil microbial diversity were studied.Three kinds of soil samples were collected,which were high-sulfur coal seam(7 cm thick)and soil below(0-10 cm)and soil not covered by high-sulfur coal nearby(0-10 cm).Soil physical and chemical properties and microbial diversity were studied to explore the changes of soil physical and chemical properties and microbial diversity under high sulfur coal cover and the correlation between environmental factors and soil microbial diversity.The results showed that:(1)High sulfur coal mulching reduced the soil pH value,increased the contents of total organic matter,sulfate,nitrogen,phosphorus and other nutrients,whilethe content of available potassium did not change much.(2)Compared with the control soil,High sulphur coal mulching increases the number of culturable bacteria in the soil beneath it,which may be due to the influence of coal seam mulching.The soil is rich in nutrients,and the pH value is about 7.0,which is more suitable for the growth of bacteria.(3)Compared with the control soil,the Alpha diversity index of microorganisms in the soil below the high sulphur coal cover was decreased.Under the influence of high sulphur coal seam,the composition of microbial community in the soil beneath high sulphur coal seam changed greatly compared with the control soil.(4)Desulfurization bacteria play a dominant role in microbial diversity in high sulphur coal seams.Proteobacteria and Firmicutes(take a total of 40.28 percent,Alphaproteobacteria and Gamma proteobacteria(take a total of 23.97percent),Acidiphilium and sulfobacillus(take a total of 22.95 percent),the abundance in the three sample groups showed that high sulfur coal seam > soil under high sulfur coal seam > soil without high sulfur coal cover,which confirmed the dominant position of desulphurization and acid-producing bacteria in high sulfur coal seam.