Effects Of Graphene On The Growth Of Raspberry And Applicatio In The Control Of Plant Fusarium Wilt

In recent years,the application of graphene in agriculture and forestry has gradually become a research hotspot.Graphene can not only promote the rapid growth of plants,but also significantly inhibit the reproduction of many common pathogens in agriculture and forestry.However,the mechanism of graphene promoting plant growth and inhibiting pathogens remains unclear.In this paper,the physiological effects of graphene on the growth and reproduction of Fusarium oxysporum and raspberry were systematically studied,and the mechanism of graphene on the growth and reproduction of the two fungi was elucidated.The main research contents are as follows:1.Effect of Graphene on the Growth and Development of Raspberry Tissue Culture SeedlingsThe effects of different concentrations of graphene on the growth of shoots above ground and adventitious roots in underground parts of raspberry tissue culture seedlings were studied.LA-S root analysis system and scanning electron microscopy were used to systematically characterize the seedling height,root length,root specific surface area,bifurcation number,root tip number and root micro-morphology of tissue-cultured seedlings.The optimum graphene concentration for the growth of tissue-cultured seedlings of raspberry was determined.The results showed that with the increase of graphene concentration,the seedling height,root length,root tip number and root specific surface area of raspberry increased first and then decreased,and the optimum concentration of graphene was 2 mg/L.When graphene was applied at the optimum concentration,the seedling height of the experimental group was 1.46 times higher than that of the control group,and the root length,specific surface area,number of root tips and bifurcation number of the experimental group were about twice as much as that of the control group,which indicated that appropriate concentration of graphene had a significant promoting effect on the growth of raspberry.2.Study on the Mechanism of Graphene Promoting the Growth and Development of RaspberryThe structure of raspberry root and the content of IAA were detected by X-ray three-dimensional microscopy and high performance liquid chromatography.The results showed that the vascular tissue of raspberry roots was more developed after adding graphene than that of control group(CK).With the increase of graphene concentration,IAA content in root tips showed a "V" trend of decreasing first and then increasing.It indicated that graphene played a certain role in promoting the growth of raspberry,and no characteristic peaks of graphene were observed in Raman detection of roots,stems and leaves of raspberry,indicating that graphene did not enter the plant interior,but affected the hormone IAA by regulating the external environment of plant roots.When IAA content in root tip is low,it can promote root vascular development and create good conditions for plants to better absorb nutrients.3.Study on the Control of Plant Fusarium Wilt by Graphene and Its MechanismThe inhibitory effect of graphene with different concentration on Fusarium oxysporum was studied by medium culture method,and its mechanism was discussed.Graphene has two main inhibitory effects on Fusarium oxysporum.Firstly,graphene surface contains a certain amount of-COOH and-OH,which can change the growth environment of Fusarium oxysporum and make its pH range from 4.2 to 5.3,which is lower than the optimum growth environment of Fusarium oxysporum(pH=5~8);secondly,graphene lamella is about 40 nm in diameter and can adhere to fungi.On the surface of somatic cell membrane,the cell membrane heaves up and destroys the cell structure,thus inhibiting the spore germination of Fusarium oxysporum.In this paper,the bacteriostasis mechanism of graphene was studied in order to find an ideal and comprehensive control method for plant Fusarium wilt control,and to open up new ideas for the application of graphene in biological field.In conclusion,graphene can promote the growth of raspberry seedlings by utilizing graphene with large specific surface area and strong adsorption capacity,which can effectively promote the absorption of nutrients by roots.Change the content of IAA in plant and promote the vascular development of plant roots.The bacteriostasis mechanism of graphene is that graphene lamellae are large in diameter and can adhere to the surface of cell membrane,causing cell membrane to warp up,destroying cell structure and inhibiting spore germination of Fusarium oxysporum.Graphene-loaded graphene synergistic compound fertilizer with traditional nutrient elements(nitrogen,phosphorus,potassium,etc.)and micronutrient elements(copper,zinc,iron,molybdenum,etc.)was developed by utilizing the promoting effect of graphene on plant growth and bacteriostasis of common diseases in agriculture and forestry.The application potential of graphene in the field of disease control in agriculture and forestry was developed,and a new green and efficient graphene synergistic fertilizer was formed.Compound fertilizer and graphene compound pesticide technology can iterate or reduce the use of traditional chemical pesticides to achieve sustainable green development of agriculture and forestry.