Comparative Analysis Of Soil Microbiota Along Degradated Mosses In The Dolomite Karst Areas,Shibing(Guizhou)

The aim of the study was to explore the relationship between mosses degradation and the structure of soil microbiota in dolomite karst areas.Here we compared the microbial community composition variation between soil covered with mosses and bare soil.In addition,we explored the effect of soil properties on different microbial communities.The co-occurrence network between differential genus was further analyzed.The aim of this study is to provide a reference for the further study of the effects of mosses degradation on soil microbial communities in dolomite karst areas.A detailed characterization of the soil microbiota of the two micro-habitats?soil covered with mosses and bare soil?by deep sequencing,using 16S rRNA V3-V4 and ITS1 regions of microorganisms.The diversity and composition of microbial community from two micro-habitats were compared.Analyzing the relationship between soil microbial communities and soil properties.In addition,we discussed the relationship between differential microbial genera based on Spearman correlation coefficient.We found that microbial communities vary significantly in two micro-habitats,and as the degeneration of mosses,the?-diversity of bacteria decreased significantly.43 bacterial phyla and 7 fungal phyla abundance in the soil microbiota.The dominant bacterial phyla included Proteobacteria,Acidobacteria and Actinobacteria,which occupied more than 60%of the total abundance of bacterial communities.The dominant fungal phyla included Zygomycota,Basidiomycota and Ascomycota,which account for more than 97%of the total abundance of fungal communities.378 bacterial genera and 594 fungal genera abundance in the soil microbiota.Moreover,the abundance of 65 bacterial and 40fungal genera varied between two micro-habitats.EMg,NH₄-N and SO₄^2-were the environmental factors that had the greatest influence on soil microbial communities.NH₄-N and SO₄^2-were negatively correlated with13 key bacterial communities,such as Mycobacterium,Marmoricola,Gaiella,while EMg was positively correlated with them.NH₄-N was positively correlated with 12 fungal communities which included Cladorrhinum,Purpureocillium,while EMg was negatively correlated with them.SO₄^2-were positively correlated with 9 key fungal communities,such as Asterostroma,Chaetospermum.Furthermore,analyzing the co-occurrence network of the differential genera showed the interaction between 7 fungal genera except Spizellomyces and bacterial genera in the soil microbiota,with the degradation of mosses.As the core genera in the co-occurrence network,Roseiflexus,Gaiella,Euzebya,Candidatus_Nitrososphaera,Corynespora and Blastococcus played a very important regulation.They interacted with each other to regulate the microbial communities changes significantly related to mosses degradation,and provided important microbial information for mosses degradation.The degradation of mosses in dolomite karst areas of Shibing,caused significant changes in the composition of soil microbial communities and significantly reduced the richness and uniformity of bacterial communities.Meanwhile,during this degradation process,soil properties(EMg,NH₄-N and SO₄^2-)played a very important role in the change of microbial community structure.Further network analysis revealed fungal and bacterial communities were mutually beneficial and competitive for adapting to changes of the environment,during the process of mosses degradation.