Construction Of Rhodol-HBT Probe And Its Colorimetric Detection Of UA Under Cu²⁺ Synergy

Involved in many physiological and pathological processes in living organisms,small biomolecules have always been the key factors to human disease detection and analysis in life analysis.Uric acid?UA?,as one of the small biomolecules,plays an important role in the human body.UA can be produced by foods containing purine nucleotides,dissolved in the blood,and excreted by urine.While the level of UA in body fluids increased,it will cause precipitation of the lower limbs and the kidneys.High levels of uric acid in the blood deposit as solid crystals in the joints of the body,causing inflammation and a very painful condition called gout or arthritis.In addition,it can also lead to kidney stones or kidney failure,Lesch-Nyhan syndrome,obesity,diabetes,high cholesterol and heart disease.In another aspect,low levels of uric acid can also cause various diseases such as liver disease,Fanconi syndrome,renal tubular disorders,cancer,Parkinson's disease and the like.Therefore,the rapid detection and accurate determination of UA concentration in body fluids is a very urgent and challenging research topic.At present,the methods of uric acid detection mainly focus on the fields of electrochemical technology and medicine.However,due to the potential interference of ascorbic acid,the high cost of the enzyme,the need to skilled experts,the operating of these techniques became complicated and laborious.Therefore,there is a great need for the detection of UA by developing simple,accurate,non-enzymatic,and reliable chemical probes.Thanks to its accurate detection capabilities,high sensitivity and fast response,chemical probes are ideal for monitoring trace amounts of extremely toxic substances in food,environmental and biological samples.The work of this paper is to use the Rhodol-HBT dye with"on-off"type,which can be converted between fluorescent?color?and non-fluorescent?colorless?,and transition metals have been widely used in the design of"on-off"probes.A carefully selected fluorophore is first coordinated to the transition metal ion to produce a fluorescent quenching complex?colorless?.The detection is in a state of fluorescence?color?in the coordination of the transition metal center or in various chemical reactions.Laid the foundation for our research.1.A Cu²⁺fluorescent"off-on"probe RHTP was designed and synthesized using the Rhodol-HBT dye fluorophore.After adding Cu²⁺to the RHTP EtOH solution system,the maximum absorption wavelength in the previous absorption spectrum is at 340 nm,and a new absorption peak of 565 nm appears.The solution changes from colorless to purple,and the fluorescence at 515 nm in the fluorescence spectrum.The intensity is weakened,showing a weak green fluorescence.The probe exhibits high selectivity and sensitivity to copper ions,a low detection limit?0.50??44??,and good colorimetric response.And successfully applied to the detection of uric acid.2.The newly synthesized probe RHTP is combined due to its fluorescent?color?and non-fluorescent?colorless?changes.On this basis,copper ions are introduced into the ethanol solution to form a uric acid detection solution capable of detecting uric acid solution UATS.After adding UA to the solution system of UATS(20??44?RHTP and 20??44?Cu²⁺,absolute ethanol),the maximum absorption at 565 nm in the absorption spectrum is weakened,the solution changes from purple to colorless,and the solution UATS pairs The response time of UA is 4 min,so it can be used for rapid detection,and it shows high selectivity and high sensitivity to UA.The detection limit is 0.06??44?.In addition,it was successfully used for the study of actual samples of serum and urine.